2-Amino-3-benzoyl thiophenes have been widely reported to act as allosteric enhancers at the A1 adenosine receptor. Their activity can be increased considerably by appropriate substitutions at the 4- and 5-positions of the thiophene ring. Substituent size at the thiophene C-4 position seemed to be a factor closely related to activity, with the 4-neopentyl (2,2-dimethylpropyl) substitution showing the greatest enhanced activity. A wide series of 2-amino-3-aroyl-4-neopentylthiophene derivatives with general structure 3, characterized by the presence of different substituents (bromine, aryl and heteroaryl) at the 5-position of the thiophene ring, have been identified as potent AEs at the A1AR. With only one exception, all of the synthesized compounds proved to be superior to the reference compound PD 81,723 in a functional assay. Replacement of the phenyl with a pyridine was detrimental for activity, with no difference in activity between the three isomeric pyridine derivatives. Derivatives 3p, 3u, 3am, 3ap and 3ar were the most active compounds in binding (saturation and competition) and functional cAMP studies, being able to potentiate agonist [3H]CCPA binding to the A1 receptor.

Synthesis and Biological Evaluation of Novel 2-Amino-3-Aroyl-4-Neopentyl-5-Substituted Thiophene Derivatives as Allosteric Enhancers of the A1 Adenosine Receptor

ROMAGNOLI, Romeo;BARALDI, Pier Giovanni;SAPONARO, Giulia;PRETI, Delia;AGHAZADEH TABRIZI, Mojgan;BARALDI, Stefania;VINCENZI, Fabrizio;VARANI, Katia
2014

Abstract

2-Amino-3-benzoyl thiophenes have been widely reported to act as allosteric enhancers at the A1 adenosine receptor. Their activity can be increased considerably by appropriate substitutions at the 4- and 5-positions of the thiophene ring. Substituent size at the thiophene C-4 position seemed to be a factor closely related to activity, with the 4-neopentyl (2,2-dimethylpropyl) substitution showing the greatest enhanced activity. A wide series of 2-amino-3-aroyl-4-neopentylthiophene derivatives with general structure 3, characterized by the presence of different substituents (bromine, aryl and heteroaryl) at the 5-position of the thiophene ring, have been identified as potent AEs at the A1AR. With only one exception, all of the synthesized compounds proved to be superior to the reference compound PD 81,723 in a functional assay. Replacement of the phenyl with a pyridine was detrimental for activity, with no difference in activity between the three isomeric pyridine derivatives. Derivatives 3p, 3u, 3am, 3ap and 3ar were the most active compounds in binding (saturation and competition) and functional cAMP studies, being able to potentiate agonist [3H]CCPA binding to the A1 receptor.
2014
Romagnoli, Romeo; Baraldi, Pier Giovanni; Carrion, M. D.; Cruz Lopez, O.; Lopez Cara, C.; Kimatrai Salvador, M.; Saponaro, Giulia; Preti, Delia; AGHAZADEH TABRIZI, Mojgan; Baraldi, Stefania; Moorman, A. R.; Vincenzi, Fabrizio; Borea, A. P.; Varani, Katia
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1901211
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 13
  • ???jsp.display-item.citation.isi??? 13
social impact