Aims of the present study were to (a) produce and characterize niosomes for possible vaginal administration of biotech drugs with antiherpetic activity, namely DTK and gB1s and (b) evaluate the activity of niosome vesicles for the administration of both peptide and secreted glycoprotein as vaccine in the vaginal infection of mouse model. Niosomes, were chosen as drug delivery systems since they may be inexpensive alternatives, of non-biological origin, to liposomes. These vesicles appear to be similar in terms of their physical properties to liposomes, being prepared in the same way and under a variety of conditions, forming unilamellar or multilamellar structures, but are characterized by a higher chemical stability with respect to liposomes, due to the difference between surfactants and phospholipids. As drug a polylysine rich peptide (DTK) derived from the herpes simplex virus type 1 (HSV-1), was employed. This peptide (DTKPKKNKKPKNPPP) belongs to the extracytoplasmatic region of glycoprotein gB involved in the recognition of a cellular receptor, namely heparan sulfate (HS) proteoglycans, and have been shown to mediate the first binding of the virion to the cell. This polylysine rich sequence, has demonstrated to possibly induce neutralizing antibody when injected into rabbits and therefore could be considered a promising immunogen for the development of a new formulated vaccine against HSV infection.
Niosomes for vaginal administration of an antiherpetic peptide
CORTESI, Rita;MENEGATTI, Enea;MANSERVIGI, Roberto;ESPOSITO, Elisabetta
2008
Abstract
Aims of the present study were to (a) produce and characterize niosomes for possible vaginal administration of biotech drugs with antiherpetic activity, namely DTK and gB1s and (b) evaluate the activity of niosome vesicles for the administration of both peptide and secreted glycoprotein as vaccine in the vaginal infection of mouse model. Niosomes, were chosen as drug delivery systems since they may be inexpensive alternatives, of non-biological origin, to liposomes. These vesicles appear to be similar in terms of their physical properties to liposomes, being prepared in the same way and under a variety of conditions, forming unilamellar or multilamellar structures, but are characterized by a higher chemical stability with respect to liposomes, due to the difference between surfactants and phospholipids. As drug a polylysine rich peptide (DTK) derived from the herpes simplex virus type 1 (HSV-1), was employed. This peptide (DTKPKKNKKPKNPPP) belongs to the extracytoplasmatic region of glycoprotein gB involved in the recognition of a cellular receptor, namely heparan sulfate (HS) proteoglycans, and have been shown to mediate the first binding of the virion to the cell. This polylysine rich sequence, has demonstrated to possibly induce neutralizing antibody when injected into rabbits and therefore could be considered a promising immunogen for the development of a new formulated vaccine against HSV infection.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
