Biological effects on human lymphocytes in vitro sonicated with a probe excited either with capacitor discharge or with bursts

Human lymphocytes were sonicated "in vitro" with short acoustical repetitive pulses, produced by a diagnostical transducer, either driven by capacitor discharge devices or by burst generators. Different biological effects were found, supported also by experimental data on pure cytochrome c reduction. Such effects prompt us to hypothesize the presence, in the acoustical pulses produced by the trasducer, of high frequency components hard to detect because of the difficulty to obtain precisely the electroacoustical transfer function of the commonly employed receiving transducers.