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EnglishThe interaction of alpha-actinin from chicken gizzard with F-actin is quite complex. The apparent dissociation constant, C, increases with the increase of actin concentration according to the following expression: C = Ko + a[actin] - c[actin]5/2. At pH 7.5 and 37 degrees C, in the presence of 0.1 M KCl and 2 mM MgCl2, the dissociation constant at infinite actin dilution, Ko, is 2.17 microM. The binding of alpha-actinin to actin is related by the term a[actin] to the diffusion of actin filaments and by the term c[actin]5/2 to the crossing number concentration of the F-actin network. Especially at low actin concentration, the binding of alpha-actinin to actin is increased by gelsolin, which fragments actin filaments and increases their diffusion. The different binding isotherms of alpha-actinin to actin filaments and to actin bundles are discussed.
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| Data di pubblicazione: | 1993 | |
| Titolo: | DIFFUSION HINDRANCE AND GEOMETRY OF FILAMENT CROSSINGS ACCOUNT FOR THE COMPLEX INTERACTIONS OF F-ACTIN WITH ALPHA-ACTININ FROM CHICKEN GIZZARD | |
| Autori: | E GRAZI; P CUNEO; E MAGRI; C. SCHWIENBACHER; G TROMBETTA | |
| Rivista: | BIOCHEMISTRY | |
| Abstract: | The interaction of alpha-actinin from chicken gizzard with F-actin is quite complex. The apparent dissociation constant, C, increases with the increase of actin concentration according to the following expression: C = Ko + a[actin] - c[actin]5/2. At pH 7.5 and 37 degrees C, in the presence of 0.1 M KCl and 2 mM MgCl2, the dissociation constant at infinite actin dilution, Ko, is 2.17 microM. The binding of alpha-actinin to actin is related by the term a[actin] to the diffusion of actin filaments and by the term c[actin]5/2 to the crossing number concentration of the F-actin network. Especially at low actin concentration, the binding of alpha-actinin to actin is increased by gelsolin, which fragments actin filaments and increases their diffusion. The different binding isotherms of alpha-actinin to actin filaments and to actin bundles are discussed. | |
| Handle: | http://hdl.handle.net/11392/463086 | |
| Appare nelle tipologie: | 03.1 Articolo su rivista |
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