Our work describes an application of a STR multiplex systems for 13 loci, previously developed and validated in our laboratory following international guidelines, on 4 cannabis specimens seized by Anti-Narcotic Italian Police. STR loci were chosen from the set of publicly available STRs for cannabis sativa and among the highly polymorphic available in literature. Gas chromatography with a flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS) analysis revealed and confirmed four comparable molecular spectrums, approximatively descriptive of the same plant/seizure origin. Due to these uncertain results, investigative authorities approved the genetic analysis. DNA profiling allowed to identify the same genetic profile and consequently the same origin and individualization of the specimens analysed.

Application of 13 loci STR multiplex for the cannabis sativa genotyping

M. Fabbri
Primo
Writing – Original Draft Preparation
;
P. Frisoni
Secondo
Methodology
;
M. Marti
Supervision
;
A. Talarico
Membro del Collaboration Group
;
O. Bonato
Membro del Collaboration Group
;
M. Coppone
Membro del Collaboration Group
;
E. Lucenti
Membro del Collaboration Group
;
R. M. Gaudio
Penultimo
Supervision
;
M. Neri
Ultimo
Writing – Review & Editing
2019

Abstract

Our work describes an application of a STR multiplex systems for 13 loci, previously developed and validated in our laboratory following international guidelines, on 4 cannabis specimens seized by Anti-Narcotic Italian Police. STR loci were chosen from the set of publicly available STRs for cannabis sativa and among the highly polymorphic available in literature. Gas chromatography with a flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS) analysis revealed and confirmed four comparable molecular spectrums, approximatively descriptive of the same plant/seizure origin. Due to these uncertain results, investigative authorities approved the genetic analysis. DNA profiling allowed to identify the same genetic profile and consequently the same origin and individualization of the specimens analysed.
Cannabis sativa, plant DNA, forensic botany
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/2417682
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