To define a prevention strategy aimed at inducing risk factors modification for cardiovascular disease (CVD), we evaluated the effect of diet therapy on hemostatic, metabolic and inflammatory parameters. Forty-nine middle-aged premenopausal women were screened for high-sensitivity C-reactive protein levels ‡1 mg/L (two independent assessments), and at least one additional CVD risk factor. Sixteen (43–54 years) were selected and received a 12-week diet (4-phases) integrating Mediterranean-style diet with National Cholesterol Education Program III guidelines. Results: Antigen levels of coagulation factor VII (P = 0.003) and VIII (P = 0.005) were remarkably reduced by intervention (maximal reduction 15.2% and 15.1%, respectively) and similar trend were observed for FVII activity levels (P = 0.048). A reduction trend in tissue factor levels was observed. At variance, we did not observe changes in fibrinogen levels and in von Willebrand factor antigen levels, which however were correlated to FVIII levels (r = 0.58 P = 0.018). Among the several inflammation markers investigated, TNF-alpha levels correlated with FVIII levels, particularly before the intervention (r = 0.55 P = 0.032), and influenced their variation over time. The functional effect of diet on coagulation, measured at low TF and phospholipid concentrations, was indicated by prolonged thrombin generation initiation, and propagation times (lag time P = 0.002; time to peak P = 0.005) but not on the total amount of generated thrombin (ETP P = 0.897). Clinically relevant reductions of body composition (BMI P = 0.001;waist circumference P = 0.005), and lipid (Total-Cholesterol P = 0.011;LDL-cholesterol P = 0.035) were observed. Conclusions: The remarkable changes observed suggest monitoring of coagulation parameters as an informative tool for evaluating shortterm effects of diet therapy. Our observations also point towards new relationships between coagulation and inflammatory components.

Modulation of coagulation factor levels and thrombin generation parameters by a healthy diet in premenopausal middle-aged women with moderate cardiovascular risk.

CALZAVARINI, Sara;PASSARO, Angelina;FELLIN, Renato;BERNARDI, Francesco
2009

Abstract

To define a prevention strategy aimed at inducing risk factors modification for cardiovascular disease (CVD), we evaluated the effect of diet therapy on hemostatic, metabolic and inflammatory parameters. Forty-nine middle-aged premenopausal women were screened for high-sensitivity C-reactive protein levels ‡1 mg/L (two independent assessments), and at least one additional CVD risk factor. Sixteen (43–54 years) were selected and received a 12-week diet (4-phases) integrating Mediterranean-style diet with National Cholesterol Education Program III guidelines. Results: Antigen levels of coagulation factor VII (P = 0.003) and VIII (P = 0.005) were remarkably reduced by intervention (maximal reduction 15.2% and 15.1%, respectively) and similar trend were observed for FVII activity levels (P = 0.048). A reduction trend in tissue factor levels was observed. At variance, we did not observe changes in fibrinogen levels and in von Willebrand factor antigen levels, which however were correlated to FVIII levels (r = 0.58 P = 0.018). Among the several inflammation markers investigated, TNF-alpha levels correlated with FVIII levels, particularly before the intervention (r = 0.55 P = 0.032), and influenced their variation over time. The functional effect of diet on coagulation, measured at low TF and phospholipid concentrations, was indicated by prolonged thrombin generation initiation, and propagation times (lag time P = 0.002; time to peak P = 0.005) but not on the total amount of generated thrombin (ETP P = 0.897). Clinically relevant reductions of body composition (BMI P = 0.001;waist circumference P = 0.005), and lipid (Total-Cholesterol P = 0.011;LDL-cholesterol P = 0.035) were observed. Conclusions: The remarkable changes observed suggest monitoring of coagulation parameters as an informative tool for evaluating shortterm effects of diet therapy. Our observations also point towards new relationships between coagulation and inflammatory components.
thrombin generation, healthy diet, premenopausal middle-aged women, cardiovascular risk.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/2367602
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