The aim of this work has been the study of the diffusion properties of the adducts between heptakis (6-deoxy-6-thio) ciclomaltoheptatose-capped gold nanoparticles (Au/β-CDSH NPs) and molecules of biological interest. In particular, we focused on 2'-deoxycytidine (DC), a nucleoside whose analogues have been used as potential anticancer and/or antiviral agents and doxorubicin hydrochloride (DOXO), a drug commonly used for the treatment of a wide range of cancers. The diffusion of both individual components and their mixtures has been studied to evaluate the interactions between capped nanoparticles and biologically active molecules. This information is important to assess the use of Au/β-CDSH NPs as medium for the transportation and the controlled release of drugs in the development of new strategies for drug delivering [1]. Experimental measurements were carried out by capillary electrophoresis (CE) . Diffusion experiments were performed by using two injection modes, either solute pulse (where an hydrodynamic injection of the sample is followed by the application of a mobilization pressure) or solute front (where mobilization pressure is applied directly to the sample). Non-ideality of CE experiment were accounted for. Taylor dispersion theory (TDA) has been employed for data interpretation [2]. Diffusion coefficients obtained for the adduct between Au/β- CDSH NPs and DC have found to be in good agreement with values obtained with other techniques [3]. This shows that TDA-CE) is an effective technique for the determination of the diffusion coefficients of NPs and their adducts. Moreover, these experiments allow for the estimation of: (a) the hydrodynamic radius of NPs and their adducts; (b) the bounded fraction of biomolecules (DC and DOXO) onto NPs and (c) the formation constant of the adducts. [1] K. Cho, X. Wang, S. Nie, Z. Chen, D. M. Shin, Clin. Cancer Res. 14(5) (2008) 1310-1316. [2] U. Sharma, N. J. Gleason, J. D. Carbeck, Anal. Chem. 77 (2005) 806-813. [3] G. Uccello-Barretta, C. Evangelisti, F. Balzano, L. Vanni, F. Aiello, L. Jicsinszky, Carbohydrate Research 346 (2011) 753-758.
CHARACTERIZATION OF ADDUCTS BETWEEN CYCLODEXTRINCAPPED GOLD NANOPARTICLES AND BIOMOLECULES BY TAYLOR DISPERSION ANALYSIS AND CAPILLARY ELECTROPHORESIS
BOSI, Valentina;SARTI, Elena;PASTI, Luisa;CAVAZZINI, Alberto
2015
Abstract
The aim of this work has been the study of the diffusion properties of the adducts between heptakis (6-deoxy-6-thio) ciclomaltoheptatose-capped gold nanoparticles (Au/β-CDSH NPs) and molecules of biological interest. In particular, we focused on 2'-deoxycytidine (DC), a nucleoside whose analogues have been used as potential anticancer and/or antiviral agents and doxorubicin hydrochloride (DOXO), a drug commonly used for the treatment of a wide range of cancers. The diffusion of both individual components and their mixtures has been studied to evaluate the interactions between capped nanoparticles and biologically active molecules. This information is important to assess the use of Au/β-CDSH NPs as medium for the transportation and the controlled release of drugs in the development of new strategies for drug delivering [1]. Experimental measurements were carried out by capillary electrophoresis (CE) . Diffusion experiments were performed by using two injection modes, either solute pulse (where an hydrodynamic injection of the sample is followed by the application of a mobilization pressure) or solute front (where mobilization pressure is applied directly to the sample). Non-ideality of CE experiment were accounted for. Taylor dispersion theory (TDA) has been employed for data interpretation [2]. Diffusion coefficients obtained for the adduct between Au/β- CDSH NPs and DC have found to be in good agreement with values obtained with other techniques [3]. This shows that TDA-CE) is an effective technique for the determination of the diffusion coefficients of NPs and their adducts. Moreover, these experiments allow for the estimation of: (a) the hydrodynamic radius of NPs and their adducts; (b) the bounded fraction of biomolecules (DC and DOXO) onto NPs and (c) the formation constant of the adducts. [1] K. Cho, X. Wang, S. Nie, Z. Chen, D. M. Shin, Clin. Cancer Res. 14(5) (2008) 1310-1316. [2] U. Sharma, N. J. Gleason, J. D. Carbeck, Anal. Chem. 77 (2005) 806-813. [3] G. Uccello-Barretta, C. Evangelisti, F. Balzano, L. Vanni, F. Aiello, L. Jicsinszky, Carbohydrate Research 346 (2011) 753-758.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
