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|Titolo:||Direct chiral resolution of underivatized amino acids on a reversed-phase stationary phase dynamically modified with the ion-exchangerNτ-decyl-l-spinacine|
|Autori interni:||REMELLI, Maurizio|
|Data di pubblicazione:||2015|
|Rivista:||JOURNAL OF SEPARATION SCIENCE|
|Abstract:||Increasing attention has been devoted in the last decades to chiral chromatography, principally to the HPLC techniques using a chiral stationary phase. Many chiral HPLC columns are commercially available, but, unfortunately, they are most often rather expensive. A cheap alternative to the commercial chiral columns is the dynamic-coating procedure of a standard achiral stationary phase with a chiral selector containing both a chiral domain and a chain or a group able to tightly (but non- covalently) bind the achiral support. This is the case of Nτ-decyl-L-spinacine, already successfully employed to dynamically cover a reversed-phase column in order to separate racemic mixtures of amino acids through the Ligand–Exchange mechanism. In the present work, the same chiral selector is employed to separate racemic mixtures of amino acids and oligopeptides, in the absence of metal ions: no coordination complex is formed, but only electrostatic and weak non-bonding interactions, between the chiral phase and the analytes, are responsible for the observed enantioselectivity. The new method is simpler than the previous one, very effective in the case of aromatic amino acids and oligopeptides and also suitable for preparative purposes.|
|Appare nelle tipologie:||03.1 Articolo su rivista|
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