Background: Sinonasal polyposis (CRSwNP) is a chronic inflammatory pathology of nasal and paranasal cavities. Human Leukocyte Antigen (HLA)-G molecules are non-classical class I antigens with anti-inflammatory and tolerogenic properties. As most theories consider polyps to be the manifestation of chronic inflammation, there could be a possible implication of HLA-G molecules in CRSwNP. Objective: To investigate the possible correlation between CRSwNP and the production of soluble HLA-G (sHLA-G) by peripheral blood mononuclear cells (PBMCs). Methods: The study involved 22 CRSwNP patients (11 without evidence of disease after surgery (NED) and 11 with relapse (RE)) and 20 healthy subjects. The presence of sHLA-G in PBMC lypopolysacharide (LPS)-stimulated culture supernatants was analyzed. The levels of interleukin 10 (IL-10), one of the main up-regulators of sHLA-G production, were determined. Exogenous IL-10 was added to the CRSwNP PBMC cultures to reconstitute the impairment in sHLA-G production. Results: Increased IL-10 levels in LPS-activated PBMC culture supernatants were found in NED patients in comparison to healthy subjects (p=0.0184). No sHLA-G production was observed in either of the patient subgroup supernatants (p<0.0001). The addition of exogenous IL-10 showed the reconstitution of sHLA-G production in NED and in a lower amount in RE patients. Conclusions: The results demonstrate a defect in sHLA-G production in CRSwNP patients mainly related to the IL-10/HLA-G pathway. Given the anti-inflammatory functions of HLA-G molecules, this impairment could increase the susceptibility to the disease. The different sHLA-G production after exogenous IL-10 addition between NED and RE CRSwNP could represent a marker of disease severity.
Decreased production of HLA-G molecules in sinonasal polyposis.
MALAGUTTI, Nicola;AIMONI, Claudia;STIGNANI, Marina;BORIN, Michela;PASTORE, Antonio;RIZZO, Roberta;
2008
Abstract
Background: Sinonasal polyposis (CRSwNP) is a chronic inflammatory pathology of nasal and paranasal cavities. Human Leukocyte Antigen (HLA)-G molecules are non-classical class I antigens with anti-inflammatory and tolerogenic properties. As most theories consider polyps to be the manifestation of chronic inflammation, there could be a possible implication of HLA-G molecules in CRSwNP. Objective: To investigate the possible correlation between CRSwNP and the production of soluble HLA-G (sHLA-G) by peripheral blood mononuclear cells (PBMCs). Methods: The study involved 22 CRSwNP patients (11 without evidence of disease after surgery (NED) and 11 with relapse (RE)) and 20 healthy subjects. The presence of sHLA-G in PBMC lypopolysacharide (LPS)-stimulated culture supernatants was analyzed. The levels of interleukin 10 (IL-10), one of the main up-regulators of sHLA-G production, were determined. Exogenous IL-10 was added to the CRSwNP PBMC cultures to reconstitute the impairment in sHLA-G production. Results: Increased IL-10 levels in LPS-activated PBMC culture supernatants were found in NED patients in comparison to healthy subjects (p=0.0184). No sHLA-G production was observed in either of the patient subgroup supernatants (p<0.0001). The addition of exogenous IL-10 showed the reconstitution of sHLA-G production in NED and in a lower amount in RE patients. Conclusions: The results demonstrate a defect in sHLA-G production in CRSwNP patients mainly related to the IL-10/HLA-G pathway. Given the anti-inflammatory functions of HLA-G molecules, this impairment could increase the susceptibility to the disease. The different sHLA-G production after exogenous IL-10 addition between NED and RE CRSwNP could represent a marker of disease severity.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
