Advanced therapy products are available for wound healing and skin regeneration, made of biomaterials combined to skin cells (fibroblasts and/or keratinocytes). Here, a device in the form of a slab, manufactured by an easy process with limited manipulation is proposed for cell delivery to the wound bed. The system is based on the combination of calcium alginate with viable fibroblasts. Alginate scaffolds are prepared from aqueous solutions of sodium alginate jellified with calcium ions. The loaded cells remain viable inside the scaffold, although they do not proliferate. Upon extraction from the alginate matrix, they regain proliferative activity in culture. When the slab is incubated in the presence of cell culture medium, slow cell release occurs from it accompanied by progressive degradation of the jellified polymer. The released cells are viable as assessed by MTT assay. In conclusion, in wound management this product would allow exploiting the beneficial effects of delivering viable autologous fibroblasts directly to the wound site by means of a carrier that holds and concentrates them where skin regeneration is requested. At the same time the encapsulated cells would be released to facilitate their interaction with the host cells, followed by migration and proliferation.

Alginate and cell combination product for wound healing

COLOMBO, Gaia;MENEGATTI, Enea;RIMESSI, Alessandro;
2011

Abstract

Advanced therapy products are available for wound healing and skin regeneration, made of biomaterials combined to skin cells (fibroblasts and/or keratinocytes). Here, a device in the form of a slab, manufactured by an easy process with limited manipulation is proposed for cell delivery to the wound bed. The system is based on the combination of calcium alginate with viable fibroblasts. Alginate scaffolds are prepared from aqueous solutions of sodium alginate jellified with calcium ions. The loaded cells remain viable inside the scaffold, although they do not proliferate. Upon extraction from the alginate matrix, they regain proliferative activity in culture. When the slab is incubated in the presence of cell culture medium, slow cell release occurs from it accompanied by progressive degradation of the jellified polymer. The released cells are viable as assessed by MTT assay. In conclusion, in wound management this product would allow exploiting the beneficial effects of delivering viable autologous fibroblasts directly to the wound site by means of a carrier that holds and concentrates them where skin regeneration is requested. At the same time the encapsulated cells would be released to facilitate their interaction with the host cells, followed by migration and proliferation.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1690793
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