A molecular approach toward the identification of the molecular bases of thrombophilia will be carried out through the study of the cofactors of the protein CX anticoagulant pathway, protein S and coagulation factor V, the deficiences of which are the most important hereditary cause of venous thrombosis.A reverse transcript PCR procedure from platelet mRNA will be coupled with rapid screening methods and direct nucleotide sequencing. Studies of mutations underlying these deficiences will improve the detection of carriers for prevention of thrombosis. Such studies will also provide data on the relationship between protein structure and function.
Difetti molecolari della proteina S e del fattore V (cofattori nella via anticoagulante della proteina C) nelle trombofilie eredo-familiari
BERNARDI, Francesco
1994
Abstract
A molecular approach toward the identification of the molecular bases of thrombophilia will be carried out through the study of the cofactors of the protein CX anticoagulant pathway, protein S and coagulation factor V, the deficiences of which are the most important hereditary cause of venous thrombosis.A reverse transcript PCR procedure from platelet mRNA will be coupled with rapid screening methods and direct nucleotide sequencing. Studies of mutations underlying these deficiences will improve the detection of carriers for prevention of thrombosis. Such studies will also provide data on the relationship between protein structure and function.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.