Mutagenic properties of terpenes (both synthesis and plant derived) have benne tested, till now, as single molecule. A terpenes containing hydro-alcoholic solution deriving from frankincense and myrrh resins and hyssop essential oil was assayed for mutagenicity by means of Ames test. Extraction technique conducted with electromagnetic fields at room temperature permitted to obtain a solution of free active molecules that did not undergo thermal degradation and characterized by biocidal activity. In order to verify lack of mutagenic hazard in coming into contact with human, the solution was appropriately diluted and tested with Salmonella typhimurium TA98, TA1535 and YG1024 strains both in absence and in presence of metabolic system S9. For none of tested conditions a 2-fold increase of induced revertants as regards to spontaneous was registered. The ratio between induced and spontaneous His+ revertants (Mutagenic Index) always resulted of about 1.00 and no statistically significant difference could be identified comparing sample and negative control. Analogous result was obtained for dose-response curve. In conclusion, having verified that tested terpenes solution is lacking of mutagenicity on Salmonella typhimurium with and without metabolic activator, this plant extract can be safely used as biocide.
Mutagenicity evaluation with Ames test of hydro-alcoholic solution of terpenes
LUPI, Silvia;MARCONI, Susanna;GREGORIO, Pasquale
2009
Abstract
Mutagenic properties of terpenes (both synthesis and plant derived) have benne tested, till now, as single molecule. A terpenes containing hydro-alcoholic solution deriving from frankincense and myrrh resins and hyssop essential oil was assayed for mutagenicity by means of Ames test. Extraction technique conducted with electromagnetic fields at room temperature permitted to obtain a solution of free active molecules that did not undergo thermal degradation and characterized by biocidal activity. In order to verify lack of mutagenic hazard in coming into contact with human, the solution was appropriately diluted and tested with Salmonella typhimurium TA98, TA1535 and YG1024 strains both in absence and in presence of metabolic system S9. For none of tested conditions a 2-fold increase of induced revertants as regards to spontaneous was registered. The ratio between induced and spontaneous His+ revertants (Mutagenic Index) always resulted of about 1.00 and no statistically significant difference could be identified comparing sample and negative control. Analogous result was obtained for dose-response curve. In conclusion, having verified that tested terpenes solution is lacking of mutagenicity on Salmonella typhimurium with and without metabolic activator, this plant extract can be safely used as biocide.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
