Persistent recruitment of neutrophils in the bronchi of cystic fibrosis patients contributes to airway tissue damage, suggesting the importance of intervening on the expression of the neutrophil chemokine IL-8. Extracts from plants have been investigated to select components able to reduce IL-8 expression in bronchial epithelial cells challenged with P.aeruginosa. Extracts and purified components have been added to cells 24h before pro-inflammatory challenge with P.aeruginosa and IL-8 transcription was quantified in the IB3-1 CF cells in vitro. P.aeruginosa-dependent IL-8 mRNA induction was increased by Argemone mexicana and Vernonia antihelmintica whereas no significant modification of transcription was observed with Aphanamixis polystachya, Lagerstroemia speciosa and Hemidesmus indicus. Finally, inhibition of IL-8 was observed with Polyalthia longifolia (IC(50)=200mug/ml) and Aegle marmelos (IC(50)=20mug/ml). Compounds from Aegle marmelos were isolated and identified by GC-MS. No significant effect was observed with butyl-p-tolyl-sulfate, whereas the inhibition obtained with 6-methyl-4-chromanone concentration was accompanied by an anti-proliferative effect. On the contrary, 5-methoxypsoralen resulted in IL-8 inhibition at 10muM concentration, without effects on cell proliferation. In synthesis, 5-methoxypsoralen can be taken into consideration to investigate mechanisms of neutrophil chemotactic signalling and for its potential application in modulating the excessive CF lung inflammation.

Modulation of expression of IL-8 gene in bronchial epithelial cells by 5-methoxypsoralen

LAMPRONTI, Ilaria
Secondo
;
BORGATTI, Monica;BIANCHI, Nicoletta;MANCINI, Irene;GAMBARI, Roberto
Ultimo
;
2009

Abstract

Persistent recruitment of neutrophils in the bronchi of cystic fibrosis patients contributes to airway tissue damage, suggesting the importance of intervening on the expression of the neutrophil chemokine IL-8. Extracts from plants have been investigated to select components able to reduce IL-8 expression in bronchial epithelial cells challenged with P.aeruginosa. Extracts and purified components have been added to cells 24h before pro-inflammatory challenge with P.aeruginosa and IL-8 transcription was quantified in the IB3-1 CF cells in vitro. P.aeruginosa-dependent IL-8 mRNA induction was increased by Argemone mexicana and Vernonia antihelmintica whereas no significant modification of transcription was observed with Aphanamixis polystachya, Lagerstroemia speciosa and Hemidesmus indicus. Finally, inhibition of IL-8 was observed with Polyalthia longifolia (IC(50)=200mug/ml) and Aegle marmelos (IC(50)=20mug/ml). Compounds from Aegle marmelos were isolated and identified by GC-MS. No significant effect was observed with butyl-p-tolyl-sulfate, whereas the inhibition obtained with 6-methyl-4-chromanone concentration was accompanied by an anti-proliferative effect. On the contrary, 5-methoxypsoralen resulted in IL-8 inhibition at 10muM concentration, without effects on cell proliferation. In synthesis, 5-methoxypsoralen can be taken into consideration to investigate mechanisms of neutrophil chemotactic signalling and for its potential application in modulating the excessive CF lung inflammation.
2009
Nicolis, E; Lampronti, Ilaria; Dechecchi, Mc; Borgatti, Monica; Tamanini, A; Bezzerri, V; Bianchi, Nicoletta; Mazzon, M; Mancini, Irene; Giri, Mg; Riz...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1378976
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