Positively charged liposomes were prepared by using three different cationic surfactants, namely cetyl-trimethyl-ammonium bromide (CTAB), didodecyl-dimethyl-ammonium bromide (DDAB12) and dioctadecyl-dimethyl-ammonium bromide (DDAB18). A study of the parameters influencing the in vitro toxicity of cationic liposomes on cultured cell lines was performed, demonstrating the lower cytotoxicity of DDAB18 containing liposomes. In addition, the stability of λDNA complexed to cationic liposomes after exposure to serum exo- and endo-nucleases was evaluated. Our results indicated that DDAB12 and DDAB18 liposomes are able to efficiently protect DNA from degradation, thus representing a potential approach to deliver nucleic acid in vivo.
Effect of cationic liposome composition on in vitro cytotoxicity and protective effect on carried DNA
CORTESI, Rita;ESPOSITO, Elisabetta;MENEGATTI, Enea;GAMBARI, Roberto;NASTRUZZI, Claudio
1996
Abstract
Positively charged liposomes were prepared by using three different cationic surfactants, namely cetyl-trimethyl-ammonium bromide (CTAB), didodecyl-dimethyl-ammonium bromide (DDAB12) and dioctadecyl-dimethyl-ammonium bromide (DDAB18). A study of the parameters influencing the in vitro toxicity of cationic liposomes on cultured cell lines was performed, demonstrating the lower cytotoxicity of DDAB18 containing liposomes. In addition, the stability of λDNA complexed to cationic liposomes after exposure to serum exo- and endo-nucleases was evaluated. Our results indicated that DDAB12 and DDAB18 liposomes are able to efficiently protect DNA from degradation, thus representing a potential approach to deliver nucleic acid in vivo.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
