A comparison of the effect of isothiocianates and nitriles derived from some glucosinolates, namely epi-progoitrin, sinalbin, glucotropaeolin, glucocheirolin and glucoraphenin, on human erythroleukemic in vitro cultured cells was studied. Many studies have in fact evidenced that a consumption of vegetable containing glucosinolates could reduce the development of colorectal cancer. In the experimental conditions used, the production of isothiocianates and nitriles from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The obtained results demonstrated that in general nitriles are considerably less potent than the corresponding isothiocyanates in inhibiting cancer cell growth. Particularly, the isothiocianates inhibitory activity on K562 cells growth is higher in the case of products derived from epi-progoitrin, glucotropaeolin, glucoraphenin and glucocheirolin; whilst for nitriles the higher activity in inhibiting K562 cells growth is showed by sinalbin derived product. Considering the antiproliferative activity found for isothiocianates and nitriles, further studies will be aimed to the possible application of glucosinolate derived products as chemopreventive cancer agents for the reduction of colorectal cancer.
In vitro antiproliferative activity of isothiocyanates and nitriles generated by myrosinase-mediated hydrolysis of glucosinolates from seeds of cruciferous vegetables
NASTRUZZI, Claudio;CORTESI, Rita;ESPOSITO, Elisabetta;MENEGATTI, Enea;
2000
Abstract
A comparison of the effect of isothiocianates and nitriles derived from some glucosinolates, namely epi-progoitrin, sinalbin, glucotropaeolin, glucocheirolin and glucoraphenin, on human erythroleukemic in vitro cultured cells was studied. Many studies have in fact evidenced that a consumption of vegetable containing glucosinolates could reduce the development of colorectal cancer. In the experimental conditions used, the production of isothiocianates and nitriles from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The obtained results demonstrated that in general nitriles are considerably less potent than the corresponding isothiocyanates in inhibiting cancer cell growth. Particularly, the isothiocianates inhibitory activity on K562 cells growth is higher in the case of products derived from epi-progoitrin, glucotropaeolin, glucoraphenin and glucocheirolin; whilst for nitriles the higher activity in inhibiting K562 cells growth is showed by sinalbin derived product. Considering the antiproliferative activity found for isothiocianates and nitriles, further studies will be aimed to the possible application of glucosinolate derived products as chemopreventive cancer agents for the reduction of colorectal cancer.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
