The production and characterization of cationic microparticles based on Eudragit RS and cationic agents (i.e. a cationic acrylic polymer and three different cationic surfactants) for the delivery of nucleic acids is here described. It was found that morphological and dimensional characteristics of microparticles were influenced by the type and concentration of cationic agent employed and by some experimental parameters such as stirring speed, emulsifying agent and type of rotor. The desoxiribonucleotide Defibrotide (DFT) was associated to positively charged microparticles and its in vitro release kinetics from microparticles were determined. A study on the in vitro toxicity of cationic microparticles on cultured human cell line K562 was also performed, demonstrating that DDAB18 microparticles display very low cytotoxicity.
Preparation and characterization of cationic microspheres for gene delivery.
ESPOSITO, Elisabetta;CORTESI, Rita;MENEGATTI, Enea;NASTRUZZI, Claudio
1999
Abstract
The production and characterization of cationic microparticles based on Eudragit RS and cationic agents (i.e. a cationic acrylic polymer and three different cationic surfactants) for the delivery of nucleic acids is here described. It was found that morphological and dimensional characteristics of microparticles were influenced by the type and concentration of cationic agent employed and by some experimental parameters such as stirring speed, emulsifying agent and type of rotor. The desoxiribonucleotide Defibrotide (DFT) was associated to positively charged microparticles and its in vitro release kinetics from microparticles were determined. A study on the in vitro toxicity of cationic microparticles on cultured human cell line K562 was also performed, demonstrating that DDAB18 microparticles display very low cytotoxicity.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
