3H-MRE 3008F20 in rat cortex were investigated through saturation binding experiments. The synthesis and the preliminary biological evaluation of the first high affinity radioligand antagonist for the human A3 adenosine receptor, named [3H]-MRE 3008-F20 are reported. [3H]-MRE 3008-20 bound human A3 receptors expressed in CHO cells with K(D) and Bmax value of 0.82 +/- 0.08 nM and 297 +/- 28 fmol/mg of protein, respectively. [3H]-MRE 3008-F20 represents a useful tool for a further characterization of A3 adenosine receptor subtype.
Synthesis and preliminary biological evaluation of [3H]-MRE 3008F20: the first high affinity radioligand antagonist for the human A3 adenosine receptors
BARALDI, Pier Giovanni;CACCIARI, Barbara;ROMAGNOLI, Romeo;VARANI, Katia;MERIGHI, Stefania;GESSI, Stefania;BOREA, Pier Andrea;SPALLUTO, Giampiero
2000
Abstract
3H-MRE 3008F20 in rat cortex were investigated through saturation binding experiments. The synthesis and the preliminary biological evaluation of the first high affinity radioligand antagonist for the human A3 adenosine receptor, named [3H]-MRE 3008-F20 are reported. [3H]-MRE 3008-20 bound human A3 receptors expressed in CHO cells with K(D) and Bmax value of 0.82 +/- 0.08 nM and 297 +/- 28 fmol/mg of protein, respectively. [3H]-MRE 3008-F20 represents a useful tool for a further characterization of A3 adenosine receptor subtype.File in questo prodotto:
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