The starting point to explain how the main biological processes occur is the understanding of the mechanisms which control the gene expression regulation. One of the mechanisms of transcriptional regulation is operating at the promoter level. in this context, the identification and characterization of transcription factors Involved on promoter binding and regulation appears to be very important. Silencing strategies are useful methods to study the function of transcription factors (TFs) as promoter regulators, and they include: 1) decoy and 2) RNA interference (RNAi) approaches. This article describes a successful application of these two methods in the study of transcriptional regulation of human A beta H-J-J locus. Transcription factor decoy (TFD) approach was employed in order to determine the role of Sp1-related proteins; RNAi approach for upstream stimulatory transcription factor (USF) depletion was used to study the role of this factor on P1 promoter directed expression.
Gene silencing in the study of transcriptional regulation: the A beta H-J-J locus
FINOTTI, AlessiaPrimo
;BREVEGLIERI, Giulia;GAMBARI, Roberto
;FERIOTTO, GiordanaUltimo
2008
Abstract
The starting point to explain how the main biological processes occur is the understanding of the mechanisms which control the gene expression regulation. One of the mechanisms of transcriptional regulation is operating at the promoter level. in this context, the identification and characterization of transcription factors Involved on promoter binding and regulation appears to be very important. Silencing strategies are useful methods to study the function of transcription factors (TFs) as promoter regulators, and they include: 1) decoy and 2) RNA interference (RNAi) approaches. This article describes a successful application of these two methods in the study of transcriptional regulation of human A beta H-J-J locus. Transcription factor decoy (TFD) approach was employed in order to determine the role of Sp1-related proteins; RNAi approach for upstream stimulatory transcription factor (USF) depletion was used to study the role of this factor on P1 promoter directed expression.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.