The development of cellular systems for the screening of molecules able to induce the production of fetal or adult hemoglobin in crythroid cells isolated from beta-thalassemia patients is very important for the identification of molecules of interest for pharmacological therapy of thalassemia. This article reports the development of a cellular system for the identification of inducers of fetal hemoglobin preferentially acting on the human gamma-globin gene promoter. To achieve this aim, green and red fluorescence protein genes were cloned under the control of gamma-globin and beta-globin promoters, respectively. The developed K562 clones were tested for the increase of fluorescence after treatment with sodium butyrate and hydroxyurea, two well-known inducers of fetal hemoglobin.

Cellular biosensors for the identification of fetal hemoglobin inducers

BREVEGLIERI, Giulia;SALVATORI, Francesca;FINOTTI, Alessia;DESTRO, Federica;FALZONI, Simonetta;BIANCHI, Nicoletta;BORGATTI, Monica;ZUCCATO, Cristina;FERIOTTO, Giordana;GAMBARI, Roberto
2007

Abstract

The development of cellular systems for the screening of molecules able to induce the production of fetal or adult hemoglobin in crythroid cells isolated from beta-thalassemia patients is very important for the identification of molecules of interest for pharmacological therapy of thalassemia. This article reports the development of a cellular system for the identification of inducers of fetal hemoglobin preferentially acting on the human gamma-globin gene promoter. To achieve this aim, green and red fluorescence protein genes were cloned under the control of gamma-globin and beta-globin promoters, respectively. The developed K562 clones were tested for the increase of fluorescence after treatment with sodium butyrate and hydroxyurea, two well-known inducers of fetal hemoglobin.
Breveglieri, Giulia; Salvatori, Francesca; Finotti, Alessia; Bertuzzi, I; Destro, Federica; Falzoni, Simonetta; Bianchi, Nicoletta; Borgatti, Monica; Zuccato, Cristina; Feriotto, Giordana; Breda, L; Rivella, S; Gambari, Roberto
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/534244
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