Chlamydophila pneumoniae, DNA and mRNA transcripts were investigated by PCR and RT-PCR in fresc CSF and PBMC specimens co-cultured in Hep-2 cell lines and collected from 14 patients with definite RR MS and 19 patients with other inflammatory (OIND) and non-inflammatory (NIND) neurological controls. A positivity for C. pneumoniae DNA and mRNA was detected in CSF and PBMC of 9 RR MS patients (64.2%) with evidence of disease activity, whereas only 3 controls were positive for Chlamydial DNA. These preliminary findings suggest that C. pneumoniae may occur in a persistent and metabolically active state of both peripheral and intrathecal levels in MS, but not in OIND and NIND.
Chlamydophila pneumoniae DNA and mRNA transcript levels in peripheral blood mononuclear cells and cerebrospinal fluid of patients with multiple sclerosis
CONTINI, Carlo;SERACENI, Silva;CASTELLAZZI, Massimiliano;GRANIERI, Enrico Gavino Giuseppe;FAINARDI, Enrico
2008
Abstract
Chlamydophila pneumoniae, DNA and mRNA transcripts were investigated by PCR and RT-PCR in fresc CSF and PBMC specimens co-cultured in Hep-2 cell lines and collected from 14 patients with definite RR MS and 19 patients with other inflammatory (OIND) and non-inflammatory (NIND) neurological controls. A positivity for C. pneumoniae DNA and mRNA was detected in CSF and PBMC of 9 RR MS patients (64.2%) with evidence of disease activity, whereas only 3 controls were positive for Chlamydial DNA. These preliminary findings suggest that C. pneumoniae may occur in a persistent and metabolically active state of both peripheral and intrathecal levels in MS, but not in OIND and NIND.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
