There is an increasing prevalence of reduced responsiveness to glucocorticoid therapy in severe asthma and chronic obstructive pulmonary disease; however the molecular mechanism of this remains unknown. Recent studies have shown that histone deacetylase activity, which is critical to glucocorticoid function, is altered by oxidant stress and may be involved in the development of glucocorticoid insensitivity. OBJECTIVES: To determine the role of phosphoinositol-3-kinase (PI3K) in the development of cigarette smoke induced glucocorticoid insensitivity. METHODS: Wild type, PI3Kgamma knock-out and PI3Kdelta kinase dead knock-in transgenic mice were used in a model of cigarette smoke induced glucocorticoid insensitivity. Peripheral lung tissue was obtained 6 healthy non-smokers, 9 smokers with normal lung function and 8 patients with chronic obstructive pulmonary disease. MEASUREMENTS AND MAIN RESULTS: In vitro oxidative stress activates PI3K and induced a relative glucocorticoid resistance which is restored by PI3K inhibition. In vivo, cigarette smoke exposure in mice increased tyrosine nitration of histone deacetylase 2 in the lung correlating with both reduced histone deacetylase 2 activity and reduced glucocorticoid function. Histone deacetylase 2 activity and the anti-inflammatory effects of glucocorticoids were restored in PI3Kdelta kinase dead knock-in but not PI3Kgamma knock-out smoke exposed mice compared to wild types, correlating with reduced histone deacetylase 2 tyrosine nitration. Glucocorticoid receptor expression was significantly reduced in smoke exposed mice, smokers with normal lung function and in chronic obstructive pulmonary disease patients. CONCLUSION: Together these data shows that therapeutic inhibition of PI3Kdelta may restore glucocorticoid function in oxidative stress induced glucocorticoid insensitivity.

Inhibition of PI-3Kdelta restores glucocorticoid function in smoking-induced airway inflammation in mice

CARAMORI, Gaetano;CASOLARI, Paolo;PAPI, Alberto
2009

Abstract

There is an increasing prevalence of reduced responsiveness to glucocorticoid therapy in severe asthma and chronic obstructive pulmonary disease; however the molecular mechanism of this remains unknown. Recent studies have shown that histone deacetylase activity, which is critical to glucocorticoid function, is altered by oxidant stress and may be involved in the development of glucocorticoid insensitivity. OBJECTIVES: To determine the role of phosphoinositol-3-kinase (PI3K) in the development of cigarette smoke induced glucocorticoid insensitivity. METHODS: Wild type, PI3Kgamma knock-out and PI3Kdelta kinase dead knock-in transgenic mice were used in a model of cigarette smoke induced glucocorticoid insensitivity. Peripheral lung tissue was obtained 6 healthy non-smokers, 9 smokers with normal lung function and 8 patients with chronic obstructive pulmonary disease. MEASUREMENTS AND MAIN RESULTS: In vitro oxidative stress activates PI3K and induced a relative glucocorticoid resistance which is restored by PI3K inhibition. In vivo, cigarette smoke exposure in mice increased tyrosine nitration of histone deacetylase 2 in the lung correlating with both reduced histone deacetylase 2 activity and reduced glucocorticoid function. Histone deacetylase 2 activity and the anti-inflammatory effects of glucocorticoids were restored in PI3Kdelta kinase dead knock-in but not PI3Kgamma knock-out smoke exposed mice compared to wild types, correlating with reduced histone deacetylase 2 tyrosine nitration. Glucocorticoid receptor expression was significantly reduced in smoke exposed mice, smokers with normal lung function and in chronic obstructive pulmonary disease patients. CONCLUSION: Together these data shows that therapeutic inhibition of PI3Kdelta may restore glucocorticoid function in oxidative stress induced glucocorticoid insensitivity.
2009
Marwick, J. A.; Caramori, Gaetano; Stevenson, C. S.; Casolari, Paolo; Jazrawi, E.; Barnes, P. J.; Ito, K.; Adcock, I. M.; Kirkham, P.; Papi, Alberto...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/530197
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