Restriction endonuclease analyses of herpes simplex virus (HSV-1) DNA's revealed variability in the electrophoretic migration of specific Bam HI and Kpn I fragments. The variable regions of the genome map at the ends and near the ends of both L and S components, near the L-S junction regio and in middle of the S component. The variability of the fragments was demonstrated by comparing the electrophoretic mobility patterns of DNAs from plaque-purified stocks originally derived from a common parental plaque-purified virus stock. Our analyses suggest that DNA sequences contained in the variable fragments can undergo accretion or deletion. So far the variability of HSV-DNA molecules has been explained in terms of number of copies of terminally reiterated sequences. Our results may be explained by unequal crossing over, slippage, because of the presence of reiterated sequences, or by the presence of transposable elements.
The heterogeneous regions in herpes simplex virus 1 DNA
TOGNON, Mauro;CASSAI, Enzo;ROTOLA, Antonella;
1983
Abstract
Restriction endonuclease analyses of herpes simplex virus (HSV-1) DNA's revealed variability in the electrophoretic migration of specific Bam HI and Kpn I fragments. The variable regions of the genome map at the ends and near the ends of both L and S components, near the L-S junction regio and in middle of the S component. The variability of the fragments was demonstrated by comparing the electrophoretic mobility patterns of DNAs from plaque-purified stocks originally derived from a common parental plaque-purified virus stock. Our analyses suggest that DNA sequences contained in the variable fragments can undergo accretion or deletion. So far the variability of HSV-DNA molecules has been explained in terms of number of copies of terminally reiterated sequences. Our results may be explained by unequal crossing over, slippage, because of the presence of reiterated sequences, or by the presence of transposable elements.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
