The effectiveness of passively transferred antibodies directed against the secretory form of herpes simplex virus type 1 (HSV-1) glycoprotein B (gB1-s) was tested in a rabbit model of ocular HSV-1 infection. The animals were passively immunized through the intramuscular injection of a homologous polyclonal anti-gB1-s antiserum at different times from the viral ocular challenge (i.e. at -24, 0, +24 and +48 h from infection). The effects observed in this trial were compared with those obtained in an active immunization trial, in which the animals were vaccinated with gB1-s before the ocular infection with HSV-1 (large variant). The results have shown that passive immunization appears quite effective in prophylactic utilization, whereas it is less effective when performed at 24 or 48 h after inoculation. By contrast, active immunization of rabbits proved to be highly effective both in preventing the development of fatal encephalitis and in reducing the severity of corneal lesions.
Effects of the passive transfer of anti-gB antibodies in a rabbit model of HSV-1-induced keratitis
INCORVAIA, Carlo;MANSERVIGI, Roberto;PARMEGGIANI, Francesco;CASELLI, Elisabetta;CASSAI, Enzo
1995
Abstract
The effectiveness of passively transferred antibodies directed against the secretory form of herpes simplex virus type 1 (HSV-1) glycoprotein B (gB1-s) was tested in a rabbit model of ocular HSV-1 infection. The animals were passively immunized through the intramuscular injection of a homologous polyclonal anti-gB1-s antiserum at different times from the viral ocular challenge (i.e. at -24, 0, +24 and +48 h from infection). The effects observed in this trial were compared with those obtained in an active immunization trial, in which the animals were vaccinated with gB1-s before the ocular infection with HSV-1 (large variant). The results have shown that passive immunization appears quite effective in prophylactic utilization, whereas it is less effective when performed at 24 or 48 h after inoculation. By contrast, active immunization of rabbits proved to be highly effective both in preventing the development of fatal encephalitis and in reducing the severity of corneal lesions.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
