DNA samples from recurrent condylomata acu‐minata biopsies of Greek males and females were examined for the presence of human papil‐lomavirus (HPV) DNA using high‐stringency Southern blot hybridization analysis. Of the twenty‐six biopsies, 25 were positive for the HPV 6/11‐related DNA sequences, and when further analyzed with the polymerase chain reaction (PCR) the HPV‐negative biopsy was also positive for HPV 6/11 DNA. Nineteen specimens were further characterized based on their Pstl restriction endonuclease hybridization pattern. Twelve biopsies were positive for HPV 6a, one biopsy was positive for HPV 11a, and one biopsy was positive for HPV 6c DNA. Three specimens contained HPV 6/11 related DNA that gave an unusual Pstl pattern, and one specimen appeared to represent a multiple HPV infection containing HPV 6/11‐ and HPV 31/35/39‐related sequences. Finally, one sample contained a mixture of HPV 6a DNA and an HPV 6a‐like genome. Biopsies were also taken from adjacent apparently normal tissue, 0.5 cm away from the lesion, in 19 of the patients. Only one of these was found to be positive for HPV 6a DNA by Southern blot analysis. © 1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc., A Wiley Company
Typing of human papillomaviruses in condylomata acuminata from Greece
ROTOLA, Antonella;
1994
Abstract
DNA samples from recurrent condylomata acu‐minata biopsies of Greek males and females were examined for the presence of human papil‐lomavirus (HPV) DNA using high‐stringency Southern blot hybridization analysis. Of the twenty‐six biopsies, 25 were positive for the HPV 6/11‐related DNA sequences, and when further analyzed with the polymerase chain reaction (PCR) the HPV‐negative biopsy was also positive for HPV 6/11 DNA. Nineteen specimens were further characterized based on their Pstl restriction endonuclease hybridization pattern. Twelve biopsies were positive for HPV 6a, one biopsy was positive for HPV 11a, and one biopsy was positive for HPV 6c DNA. Three specimens contained HPV 6/11 related DNA that gave an unusual Pstl pattern, and one specimen appeared to represent a multiple HPV infection containing HPV 6/11‐ and HPV 31/35/39‐related sequences. Finally, one sample contained a mixture of HPV 6a DNA and an HPV 6a‐like genome. Biopsies were also taken from adjacent apparently normal tissue, 0.5 cm away from the lesion, in 19 of the patients. Only one of these was found to be positive for HPV 6a DNA by Southern blot analysis. © 1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc., A Wiley CompanyI documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
