The production and characterization of cationic microparticles based on methacrylate copolymer constituted of acrylic and methacrylic acid esters (Eudragit RS) and the cationic agent dioctadecyl-dimethyl-ammonium bromide (DDAB18) for the delivery of nucleic acids is described. It was found that morphological and dimensional characteristics of microparticles were influenced by some experimental parameters such as stirring speed, emulsifying agent and type of rotor. The deoxyribonucleotide Defibrotide (DFT) was associated to positively charged microparticles and its in vitro release kinetics from microparticles was determined. A study on the in vitro toxicity of cationic microparticles on cultured human cell line K562 was also performed which demonstrated that the cationic surfactant dioctadecyl-dimethyl-ammonium bromide (DDAB18) microparticles display very low cytotoxicity.

Oligonucleotide transport and cellular uptake by positively charged microparticles

MAZZITELLI, Stefania;CORTESI, Rita;ESPOSITO, Elisabetta;NASTRUZZI, Claudio
2007

Abstract

The production and characterization of cationic microparticles based on methacrylate copolymer constituted of acrylic and methacrylic acid esters (Eudragit RS) and the cationic agent dioctadecyl-dimethyl-ammonium bromide (DDAB18) for the delivery of nucleic acids is described. It was found that morphological and dimensional characteristics of microparticles were influenced by some experimental parameters such as stirring speed, emulsifying agent and type of rotor. The deoxyribonucleotide Defibrotide (DFT) was associated to positively charged microparticles and its in vitro release kinetics from microparticles was determined. A study on the in vitro toxicity of cationic microparticles on cultured human cell line K562 was also performed which demonstrated that the cationic surfactant dioctadecyl-dimethyl-ammonium bromide (DDAB18) microparticles display very low cytotoxicity.
Mazzitelli, Stefania; C., Balestra; Cortesi, Rita; Esposito, Elisabetta; Nastruzzi, Claudio
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11392/529766
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact