In vivo and in vitro skin permeation of butyl methoxydibenzoylmethane from lipospheres

Lipid microparticles loaded with butyl methoxydibenzoylmethane were prepared by melt technique and evaluate for skin permeation both in vivo, by tape stripping, and in vitro, by a flow-through diffusion chamber. Following in vivo human skin application of an O/W emulsion containing 2% butyl methoxydibenzoylmethane in lipospheres, 15% of the applied sunscreen accumulated in the uppermost layers of the stratum corneum without remarkably modifying the skin permeation of the unencapsulated sunscreen. These results were found to be predicted by an in vitro methodology involving the diffusion of butyl methoxydibenzoylmethane through a lipophilized synthetic membrane into a hydrophilic receptor phase, simulating the viable epidermis better than an ethanolic receptor phase