We describe the function of BK virus origin of replication present in the construction of some plasmid vectors. In human cells transfected with different recombinant DNA molecules, al containing the BK virus origin of replication, we observed an increase of the amount of the recombination DNA 36/48 hours after transfection. We aim to use these vectors to study the expression of eukoryotic genes in human cells in transient assay.
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