BK virus (BKV)-transformed cells and BKV-induced tumors as well as in vitro derived tumor cell lines were all found to contain BKV DNA sequences when analysed by DNA-DNA reassociation kinetics. In transformed cells the number of viral genome equivalents per diploid cell genome (VG/CG) decreased with increasing generations. Likewise, single cell clones had a lower VG/CG than parental transformed cells. BKV-induced tumors had a high VG/CG. Tumor cells cultivated in vitro and their clones had a lower VG/CG than BKV-induced tumors from which they were derived, suggesting a multiclonal origin of tumors. Hamster tumors induced by subcutaneous inoculation of BKV-transformed cells or tumor cell lines had a higher VG/CG than cells producing them. Variation in VG/CG is discussed in terms of cell selection depending on different in vitro or in vivo conditions of cell growth. In some cases, however, the decrease in VG/CG most likely depends on loss of free viral sequences from transformed cells or tumors. © 1981 Springer-Verlag.

Analysis of BK virus-transformed cells and BK virus-induced tumors by DNA-DNA reassociation kinetics

GROSSI, Maria Pia;CORALLINI, Alfredo;POLI, Ferruccio;BARBANTI BRODANO, Giuseppe
1981

Abstract

BK virus (BKV)-transformed cells and BKV-induced tumors as well as in vitro derived tumor cell lines were all found to contain BKV DNA sequences when analysed by DNA-DNA reassociation kinetics. In transformed cells the number of viral genome equivalents per diploid cell genome (VG/CG) decreased with increasing generations. Likewise, single cell clones had a lower VG/CG than parental transformed cells. BKV-induced tumors had a high VG/CG. Tumor cells cultivated in vitro and their clones had a lower VG/CG than BKV-induced tumors from which they were derived, suggesting a multiclonal origin of tumors. Hamster tumors induced by subcutaneous inoculation of BKV-transformed cells or tumor cell lines had a higher VG/CG than cells producing them. Variation in VG/CG is discussed in terms of cell selection depending on different in vitro or in vivo conditions of cell growth. In some cases, however, the decrease in VG/CG most likely depends on loss of free viral sequences from transformed cells or tumors. © 1981 Springer-Verlag.
1981
Grossi, Maria Pia; Corallini, Alfredo; Poli, Ferruccio; Valieri, A.; Milanesi, G.; BARBANTI BRODANO, Giuseppe
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/523304
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