BK virus (BKV)-transformed cells and BKV-induced tumors as well as in vitro derived tumor cell lines were all found to contain BKV DNA sequences when analysed by DNA-DNA reassociation kinetics. In transformed cells the number of viral genome equivalents per diploid cell genome (VG/CG) decreased with increasing generations. Likewise, single cell clones had a lower VG/CG than parental transformed cells. BKV-induced tumors had a high VG/CG. Tumor cells cultivated in vitro and their clones had a lower VG/CG than BKV-induced tumors from which they were derived, suggesting a multiclonal origin of tumors. Hamster tumors induced by subcutaneous inoculation of BKV-transformed cells or tumor cell lines had a higher VG/CG than cells producing them. Variation in VG/CG is discussed in terms of cell selection depending on different in vitro or in vivo conditions of cell growth. In some cases, however, the decrease in VG/CG most likely depends on loss of free viral sequences from transformed cells or tumors. © 1981 Springer-Verlag.
Analysis of BK virus-transformed cells and BK virus-induced tumors by DNA-DNA reassociation kinetics
GROSSI, Maria Pia;CORALLINI, Alfredo;POLI, Ferruccio;BARBANTI BRODANO, Giuseppe
1981
Abstract
BK virus (BKV)-transformed cells and BKV-induced tumors as well as in vitro derived tumor cell lines were all found to contain BKV DNA sequences when analysed by DNA-DNA reassociation kinetics. In transformed cells the number of viral genome equivalents per diploid cell genome (VG/CG) decreased with increasing generations. Likewise, single cell clones had a lower VG/CG than parental transformed cells. BKV-induced tumors had a high VG/CG. Tumor cells cultivated in vitro and their clones had a lower VG/CG than BKV-induced tumors from which they were derived, suggesting a multiclonal origin of tumors. Hamster tumors induced by subcutaneous inoculation of BKV-transformed cells or tumor cell lines had a higher VG/CG than cells producing them. Variation in VG/CG is discussed in terms of cell selection depending on different in vitro or in vivo conditions of cell growth. In some cases, however, the decrease in VG/CG most likely depends on loss of free viral sequences from transformed cells or tumors. © 1981 Springer-Verlag.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
