Sedimentation Field Flow Fractionation (SdFFF) is a well established elution technique, which has been successfully applied for the separation and the size characterization of a broad range of natural and synthetic colloidal particles of environmental, industrial, pharmaceutical and biological interest. Differential SdFFF can precisely evaluate surface concentrations of human immunoglobulin G (IgG) adsorbed to polystyrene latex. The adsorption occurs without altering the IgG reactivity, as proven by the reaction with its specific anti-human IgGs. This type of immune complex, which used for assessment of a patient’s level of immune response to a particular antigen, has been detected by SdFFF by measuring the variations in the coated PS buoyancy mass. Among with all allergic diseases, food allergy is a growing issue, which deserves the attention of always more researchers and Institutions in developed and developing countries. Although food normally doesn't provoke a response from the human immune system, in allergenic patients the hypersensivity reactions are triggered by the IgE recognition of specific sequences (epitopes) present in the allergenic proteins. Peach allergy is the most common form of IgE-mediated hypersensitivity to fresh fruits in the Mediterranean area, as well as in the USA. Several are the allergens from peach, but the major is an allergen belonging to the family of lipid-transfer proteins (LTP). The aim of this study was to use the SdFFF technique as an high-resolution technique to detect the interaction between the IgE produced by the human body in consequence of an allergic food reaction due to the ingestion of peaches and specific anti human IgEs. Preliminary tests done by using IgG, IgA and anti-IgA have proven the specificity of the surface immuno-reaction, while corollary experiments have been made to individuate good fractionation conditions.
A Sedimentation FFF study to monitor bio-interactions on surface-modified PS micro-spheres.
CONTADO, Catia;BREGOLA, Letizia;DONDI, Francesco
2007
Abstract
Sedimentation Field Flow Fractionation (SdFFF) is a well established elution technique, which has been successfully applied for the separation and the size characterization of a broad range of natural and synthetic colloidal particles of environmental, industrial, pharmaceutical and biological interest. Differential SdFFF can precisely evaluate surface concentrations of human immunoglobulin G (IgG) adsorbed to polystyrene latex. The adsorption occurs without altering the IgG reactivity, as proven by the reaction with its specific anti-human IgGs. This type of immune complex, which used for assessment of a patient’s level of immune response to a particular antigen, has been detected by SdFFF by measuring the variations in the coated PS buoyancy mass. Among with all allergic diseases, food allergy is a growing issue, which deserves the attention of always more researchers and Institutions in developed and developing countries. Although food normally doesn't provoke a response from the human immune system, in allergenic patients the hypersensivity reactions are triggered by the IgE recognition of specific sequences (epitopes) present in the allergenic proteins. Peach allergy is the most common form of IgE-mediated hypersensitivity to fresh fruits in the Mediterranean area, as well as in the USA. Several are the allergens from peach, but the major is an allergen belonging to the family of lipid-transfer proteins (LTP). The aim of this study was to use the SdFFF technique as an high-resolution technique to detect the interaction between the IgE produced by the human body in consequence of an allergic food reaction due to the ingestion of peaches and specific anti human IgEs. Preliminary tests done by using IgG, IgA and anti-IgA have proven the specificity of the surface immuno-reaction, while corollary experiments have been made to individuate good fractionation conditions.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
