Anomalies of spermatozoa of Sparus aurata and Diplodus puntazzo caused by different cryogenic protocols were analysed. Milt was diluted with cryosolutions containg different diluent (0.1 M Na citrate and 0.17 M NaCl) and cryoprotective (DMSO and glycerol). Ultrastructure was studied by TEM at different phases of the cryopreservation procedure, that induced sperm anomalies varying in quantity with the different protocols. In particular, cryosolutions with 0.1 N Na citrate or 0.17 M NaCl were equally effective when used according to the same protocol, while the relative concentration of DMSO likely determined the different incidence of anomalies detected.
Effects of cryopreservation on the fish spermatozoa ultrastructure.
ABELLI, Luigi;
2002
Abstract
Anomalies of spermatozoa of Sparus aurata and Diplodus puntazzo caused by different cryogenic protocols were analysed. Milt was diluted with cryosolutions containg different diluent (0.1 M Na citrate and 0.17 M NaCl) and cryoprotective (DMSO and glycerol). Ultrastructure was studied by TEM at different phases of the cryopreservation procedure, that induced sperm anomalies varying in quantity with the different protocols. In particular, cryosolutions with 0.1 N Na citrate or 0.17 M NaCl were equally effective when used according to the same protocol, while the relative concentration of DMSO likely determined the different incidence of anomalies detected.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.