The pathological changes induced by an infection of Diphyllobothrium dendriticum (Nitzsch, 1824) plerocercoids in powan, Coregonus lavaretus (L.), from Loch Lomond, Scotland were assessed using a range of immunohistochemical and ultrastructural techniques. From a sample of 36 powan, the occurrence of encysted plerocercoids of D. dendriticum on the outer surface of the stomach was 38.5% (n=10) with the number of cysts ranging from 4 to 15 in number and measuring 4.2 ± 1.0 mm × 3.4 ± 0.9 mm in dimensions (mean ± SD). Histological examination of intestinal samples also revealed the presence of plerocercoids (2-21) encapsulated within a proliferation of mesenteric fibrous tissues of the gastric wall and, occasionally, by the gut lamina propria-submucosa and lamina muscularis. In section, cysts were tri-layered and were formed from a series of concentric whorls of fibroblast and collagen fibre-based connective elements. The extent of necrosis within each of the muscle layers and the serosa of the stomach was found to differ, notably within the latter which was marked by a chronic inflammatory reaction and fibrosis. Within the body of the cyst and in the vicinity around it, a high number of mast cell / eosinophilic granule cells (EGCs) in a state of degranulation were seen, in addition to melano-macrophages centres (MMC). Immunohistochemical staining of histological sections of infected stomach material revealed a high density of elements, in close proximity to plerocercoids, staining positive for serotonin (5-HT), bombesin, substance P (SP) and galanin. Uninfected material did not present the same levels of activity. Sections through both infected and uninfected tissue were also tested for the presence of elements containing vasoactive intestinal peptide (VIP), met-enkephalin, calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and nitric oxide synthase (NOS), but were found to be absent.
Selected pathological, immunohistochemical and ultrastructural changes associated with an infection by Diphyllobothrium dendriticum (Nitzsch, 1824) (Cestoda) plerocercoids in Coregonus lavaretus (L.) (Coregonidae)
SAYYAF DEZFULI, Bahram;SIMONI, Edi;GIARI, Luisa
2007
Abstract
The pathological changes induced by an infection of Diphyllobothrium dendriticum (Nitzsch, 1824) plerocercoids in powan, Coregonus lavaretus (L.), from Loch Lomond, Scotland were assessed using a range of immunohistochemical and ultrastructural techniques. From a sample of 36 powan, the occurrence of encysted plerocercoids of D. dendriticum on the outer surface of the stomach was 38.5% (n=10) with the number of cysts ranging from 4 to 15 in number and measuring 4.2 ± 1.0 mm × 3.4 ± 0.9 mm in dimensions (mean ± SD). Histological examination of intestinal samples also revealed the presence of plerocercoids (2-21) encapsulated within a proliferation of mesenteric fibrous tissues of the gastric wall and, occasionally, by the gut lamina propria-submucosa and lamina muscularis. In section, cysts were tri-layered and were formed from a series of concentric whorls of fibroblast and collagen fibre-based connective elements. The extent of necrosis within each of the muscle layers and the serosa of the stomach was found to differ, notably within the latter which was marked by a chronic inflammatory reaction and fibrosis. Within the body of the cyst and in the vicinity around it, a high number of mast cell / eosinophilic granule cells (EGCs) in a state of degranulation were seen, in addition to melano-macrophages centres (MMC). Immunohistochemical staining of histological sections of infected stomach material revealed a high density of elements, in close proximity to plerocercoids, staining positive for serotonin (5-HT), bombesin, substance P (SP) and galanin. Uninfected material did not present the same levels of activity. Sections through both infected and uninfected tissue were also tested for the presence of elements containing vasoactive intestinal peptide (VIP), met-enkephalin, calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and nitric oxide synthase (NOS), but were found to be absent.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
