The influence exerted by monoamines on acetylcholine release was studied in electrically stimulated slices of guinea pig nucleus basalis magnocellularis (nbM) prelabelled with 3H-choline (3H-Ch). Noradrenaline, 30 μM, and clonidine, 1 μM, reduced the evoked 3H-Ch efflux by about 50%, but phenylephrine, 100 μM. did not; idazoxan, 0.1 μM. but not prazosin, 1 μM, antagonized these effects. pointing to the involvement of alpha2 receptors. Apomorphine, 1 or 30 μM. reduced 3H-Ch efflux from nbM slices as well. The effect was shared by quinpirole, 1 or 10 μM, but not by 2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benz-azepine (SKF 38393). 10 μM, and was antagonized by sulpiride, 1 μM, but not by R-(+)-8-chloro-2,3,4,5-tetra-hydro-3-methyl-5-phenyl-1H-3-benzazepin-7-ol (SCH 23390). 1 μM, suggesting the involvement of the D2 receptor subtype. 5-hydroxytryptamine (5-HT) 0.3-30 μM, and alphamethyl-5-HT, 10 μM, significantly increased 3H-Ch efflux from nbM slices; the 5-HT2 antagonist ritanseri...
Monoamines modulate the electrically-evoked efflux of 3H-choline from slices of guinea pig nucleus basalis magnocellularis
Siniscalchi A.;
1994
Abstract
The influence exerted by monoamines on acetylcholine release was studied in electrically stimulated slices of guinea pig nucleus basalis magnocellularis (nbM) prelabelled with 3H-choline (3H-Ch). Noradrenaline, 30 μM, and clonidine, 1 μM, reduced the evoked 3H-Ch efflux by about 50%, but phenylephrine, 100 μM. did not; idazoxan, 0.1 μM. but not prazosin, 1 μM, antagonized these effects. pointing to the involvement of alpha2 receptors. Apomorphine, 1 or 30 μM. reduced 3H-Ch efflux from nbM slices as well. The effect was shared by quinpirole, 1 or 10 μM, but not by 2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benz-azepine (SKF 38393). 10 μM, and was antagonized by sulpiride, 1 μM, but not by R-(+)-8-chloro-2,3,4,5-tetra-hydro-3-methyl-5-phenyl-1H-3-benzazepin-7-ol (SCH 23390). 1 μM, suggesting the involvement of the D2 receptor subtype. 5-hydroxytryptamine (5-HT) 0.3-30 μM, and alphamethyl-5-HT, 10 μM, significantly increased 3H-Ch efflux from nbM slices; the 5-HT2 antagonist ritanseri...I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
