Optimization of the chromatographic separation of flavonoid compounds in camomile extracts by the simplex method and by the Monte Carlo method is described. Evaluation of the number of peaks at unit resolution (Rs = 1) is used as the criterion of separation quality. The Davis-Giddings theory is applied in calculating the number of components and the results are validated by numerical simulations. Peak-purity checks for three identified compounds in commercial sample extracts are reported. Capacity factor patterns for 18 flavonoid and phenolic compounds over an extended range of methanol/aqueous buffer (pH 2.8) mobile phases are described.
Evaluation of the number of components in multicomponent liquid chromatograms of plant extracts
DONDI, Francesco;LODI, Gaetano;REMELLI, Maurizio;BIGHI, Corrado
1986
Abstract
Optimization of the chromatographic separation of flavonoid compounds in camomile extracts by the simplex method and by the Monte Carlo method is described. Evaluation of the number of peaks at unit resolution (Rs = 1) is used as the criterion of separation quality. The Davis-Giddings theory is applied in calculating the number of components and the results are validated by numerical simulations. Peak-purity checks for three identified compounds in commercial sample extracts are reported. Capacity factor patterns for 18 flavonoid and phenolic compounds over an extended range of methanol/aqueous buffer (pH 2.8) mobile phases are described.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
