Fluidic and syringe injections with different splitting conditions are compared by using the Edgeworth-Cramer peak shape analysis. Non retained component peaks on capillary and packed columns were considered. Skewness values as low as 0.02, with precision and repeatability of ±0.005, were obtained when using the capillary column with fluidic injection. Extracolumn contributions coming from the injection system were evaluated by using peak skewness under the hypothesis of exponential decay extracolumn factor. The corrected column efficiency values are in good agreement with the theoretically expected van Deemter equation data. A syringe injection system with high split rate can produce, for unretained samples, peak fidelity values greater than 0.8, which are comparable with those observed with the fluidic injection system. Applications in column performance studies are discussed.
Fluidic and syringe injection study by peak shape analysis
REMELLI, Maurizio;BLO, Gabriella;DONDI, Francesco;
1989
Abstract
Fluidic and syringe injections with different splitting conditions are compared by using the Edgeworth-Cramer peak shape analysis. Non retained component peaks on capillary and packed columns were considered. Skewness values as low as 0.02, with precision and repeatability of ±0.005, were obtained when using the capillary column with fluidic injection. Extracolumn contributions coming from the injection system were evaluated by using peak skewness under the hypothesis of exponential decay extracolumn factor. The corrected column efficiency values are in good agreement with the theoretically expected van Deemter equation data. A syringe injection system with high split rate can produce, for unretained samples, peak fidelity values greater than 0.8, which are comparable with those observed with the fluidic injection system. Applications in column performance studies are discussed.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
