Human lymphocytes sonicated in vitro with pulsed ultrasounds of the same frequency and intensity as those used far diagnostic purposes appear to cause two different types of damage of DNA molecules. One can be repaired within a short time (60 minutes) and involves single strand breaking. The other one requires a longer time far repair (20 to 24 hours) and appears to be due to the formation of large excision repairs leading to Sister Chromatid Exchange (SCE). A spectrofluorimetric method evidencing these two types of damage is described. Electrophoretic anomalies of damaged molecules are shown. along with morphological ones, by transmission electron microscopy (TEM).
Two forms of repair in the DNA of human lymphocytes damaged by ultrasound esposure in vitro.
GRANDI, Gilberto;
1990
Abstract
Human lymphocytes sonicated in vitro with pulsed ultrasounds of the same frequency and intensity as those used far diagnostic purposes appear to cause two different types of damage of DNA molecules. One can be repaired within a short time (60 minutes) and involves single strand breaking. The other one requires a longer time far repair (20 to 24 hours) and appears to be due to the formation of large excision repairs leading to Sister Chromatid Exchange (SCE). A spectrofluorimetric method evidencing these two types of damage is described. Electrophoretic anomalies of damaged molecules are shown. along with morphological ones, by transmission electron microscopy (TEM).I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
