Hepatocytes were isolated from catfish Ictalurus melas by conventional collagenase digestion. Sensitivities of liver cells from the same fish to the glycogenolytic action of epinephrine, mammalian glucagon, catfish glucagon-like peptide, synthetic fragment 19-29 of anglerfish glucagon I, fragment 19-29 of anglerfish glucagon II, and anglerfish glucagon II were compared in two different systems: perifusion in a Bio-Gel P4 column and flask incubation. The results obtained in this study indicate that piscine hepatocytes suspended and perifused in a Bio-Gel column are more sensitive to physiological concentrations of glycogenolytic hormones and may represent a new tool for experimental studies of fish liver metabolism and its hormonal regulation.
Hormone responsiveness in isolated catfish hepatocytes in perifusion system is higher than in flasks incubation
A. C. PUVIANI;CAPUZZO, Antonio;
1994
Abstract
Hepatocytes were isolated from catfish Ictalurus melas by conventional collagenase digestion. Sensitivities of liver cells from the same fish to the glycogenolytic action of epinephrine, mammalian glucagon, catfish glucagon-like peptide, synthetic fragment 19-29 of anglerfish glucagon I, fragment 19-29 of anglerfish glucagon II, and anglerfish glucagon II were compared in two different systems: perifusion in a Bio-Gel P4 column and flask incubation. The results obtained in this study indicate that piscine hepatocytes suspended and perifused in a Bio-Gel column are more sensitive to physiological concentrations of glycogenolytic hormones and may represent a new tool for experimental studies of fish liver metabolism and its hormonal regulation.File | Dimensione | Formato | |
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