A Dot Immunobinding (DIB) assay has been applied to the serodiagnosis of human hydatidosis and its results have been compared with those obtained with the enzyme-linked immunosorbent assay (ELISA). The two techniques have been shown to be closely related (p greater than 0.001), highly sensitive (92.0% of positive results in 75 sera from patients with hepatic or pulmonary hydatidosis) and specific (93.5% of negative results in 31 sera from patients affected by other parasitic diseases and 100% of negative results in 30 normal controls). DIB however is more economical and takes less time (only 4 hours) than ELISA. DIB could be an useful tool in field epidemiological surveys since it is sensitive, specific, simple to perform and it does not require any expensive apparatus.
Use of dot immunobinding assay for the rapid diagnosis of human hydatidosis.
CONTINI, Carlo;
1985
Abstract
A Dot Immunobinding (DIB) assay has been applied to the serodiagnosis of human hydatidosis and its results have been compared with those obtained with the enzyme-linked immunosorbent assay (ELISA). The two techniques have been shown to be closely related (p greater than 0.001), highly sensitive (92.0% of positive results in 75 sera from patients with hepatic or pulmonary hydatidosis) and specific (93.5% of negative results in 31 sera from patients affected by other parasitic diseases and 100% of negative results in 30 normal controls). DIB however is more economical and takes less time (only 4 hours) than ELISA. DIB could be an useful tool in field epidemiological surveys since it is sensitive, specific, simple to perform and it does not require any expensive apparatus.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
