A dot immunobinding assay (DIBA) with non-commercial monoclonal antibodies (Mabs) was developed in order to investigate the presence of P. carinii in bronchoalveolar lavage fluids (BAL) obtained from 93 Italian patients with AIDS and 13 HIV positive patients without immunological abnormalities with fever and or chest signs of bronchovascular markings and 15 HIV antibody -negative patients with haematologic malignancies without any features suggestive of PCP. IIF with Mab has been shown to be better than the cytochemical method silver stain, the advantages including time saving and earlier detection of the cysts or trophozoites. DIBA has also been shown to provide a suitable tool for detection of P. carinii with elevated sensitivity and specificity.
Detection of Pneumocystis carinii in bronchoalveolar lavage fluid by dot immunobinding assay and immunofluorescence.
CONTINI, Carlo;
1992
Abstract
A dot immunobinding assay (DIBA) with non-commercial monoclonal antibodies (Mabs) was developed in order to investigate the presence of P. carinii in bronchoalveolar lavage fluids (BAL) obtained from 93 Italian patients with AIDS and 13 HIV positive patients without immunological abnormalities with fever and or chest signs of bronchovascular markings and 15 HIV antibody -negative patients with haematologic malignancies without any features suggestive of PCP. IIF with Mab has been shown to be better than the cytochemical method silver stain, the advantages including time saving and earlier detection of the cysts or trophozoites. DIBA has also been shown to provide a suitable tool for detection of P. carinii with elevated sensitivity and specificity.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
