XXIII° Congresso Nazionale A.I.B.G. Camogli (GE). Abstract Book

As a highly aggressive primary bone malignancy, osteosarcoma (OS) presents a significant clinical challenge due to its elevated metastatic potential. Standard therapeutic protocols, comprising surgical resection and conventional chemotherapy, are severely hampered by profound adverse effects and the development of chemoresistance, highlighting the urgent requirement for alternative therapeutic strategies. To address this need, the present study explores the antineoplastic potential of the natural polyphenol resveratrol (RSV), evaluating its efficacy across distinct human osteosarcoma cell models. Human SAOS-2 and U2-OS cells were exposed to RSV. Intracellular accumulation of RSV was confirmed by non-invasive hyperspectral imaging. Functional assays were conducted to monitor cell viability (MTT), S-phase distribution, and cell motility. High-throughput transcriptional profiling was performed using specialized qRT-PCR arrays targeting apoptosis and extracellular matrix/adhesion molecules. Spatial localization of Vimentin and β- catenin signaling proteins was tracked via immunocytochemistry. RSV inhibited cell proliferation, arresting the cell cycle specifically in the S-phase and inducing caspase-dependent programmed cell death. RSV modulates gene expression of the following cellular process such as apoptosis (CASP 3, 4,7), cellular adhesion (ITGAM, ITGB2, CCN2, ADAMTS13). Expression genes of SPARC and SPP1 are down-regulated in both lines. RSV modulates the Wnt/β-Catenin signaling and inhibits cell migration in OS cells. Resveratrol-induced anti-proliferative effects and S-phase cell cycle arrest in osteosarcoma cancer cells with gene expression modulation. The regulation of genes related to cell adhesion and extracellular matrix dynamics may contribute to reducing the invasive potential and tumorigenic properties of osteosarcoma cells exposed to RSV.