Letter Regarding: “Quantitative Analysis of Endothelial Cell Damage in Corneal Grafts Used for Endothelial Keratoplasty and Correlation to Donor Tissue Characteristics and Postoperative Parameters”

We read with great interest the recent study by Scherer et al 1 on the quantitative analysis of endothelial cell damage in corneas undergoing Desce- met membrane endothelial keratoplasty (DMEK) preparation. The authors re- ported patterns of endothelial cell loss, as evidenced by trypan blue (TB) staining, in 36 donor corneas before performing a no-touch liquid bubble DMEK preparation technique. Although their meticulous work adds valuable evidence to the growing inter- est of corneal specialists in quality assurance of donor tissues, 2 we would like to underscore specific technical aspects that may have reduced the accuracy of the imaging analysis and to contextualize the authors’ findings within recent efforts to standardize pre- operative assessment of endothelial viability.