Hibiscus ssp. is a source of phytochemicals, mainly anthocyanins, widely known for its medicinal properties. The aim of this study was to develop a microwave-assisted extraction (MAE) method to extract bioactive compounds from yellow and red petals of Hibiscus rosa-sinensis L. (HRS) and evaluate their antioxidant and eye irritation potential. For MAE, the effect of microwave power, irradiation time, solvent concentration and solvent volume was tested. The antioxidant activity and eye irritation potentialwere evaluated. The extracts were analyzed by LC-DAD-ESI-IT-MS/MS. The acidified aqueous extractionwas the best choice for the recovery of anthocyanins, while hydroethanolic was best extractor solvent to recovery flavonol and flavanols. MAE extracts showed efficient recovery: total phenolic content (28.4 and23.2 mg GAE/g), total flavonoids content (44.8 and 34.5 mg RE/g), total anthocyanins content (4.1 and 16.3mg Cy-3-glu/100 g) and extraction yields (29.5 and 23.2 %) and remarkable antioxidant activity(IC50=12.35 and 15.76 mg L-1) for yellow and red petal extracts, respectively. Main compounds werecyanidin-3-sophoroside and cyanidin-3,5-diglucoside, besides catechin, epicatechin and rutin. The extractswere classified as non-irritating ingredients. The data revealed HRS as a source of bioactive compounds and significant variations in phytochemical content among yellow and red petals of HRS.
Phytochemical analysis, antioxidant activity and in vitro ocular irritation of Hibiscus rosa-sinensis L. extracts
Polidoro, Allan dos Santos;
2020
Abstract
Hibiscus ssp. is a source of phytochemicals, mainly anthocyanins, widely known for its medicinal properties. The aim of this study was to develop a microwave-assisted extraction (MAE) method to extract bioactive compounds from yellow and red petals of Hibiscus rosa-sinensis L. (HRS) and evaluate their antioxidant and eye irritation potential. For MAE, the effect of microwave power, irradiation time, solvent concentration and solvent volume was tested. The antioxidant activity and eye irritation potentialwere evaluated. The extracts were analyzed by LC-DAD-ESI-IT-MS/MS. The acidified aqueous extractionwas the best choice for the recovery of anthocyanins, while hydroethanolic was best extractor solvent to recovery flavonol and flavanols. MAE extracts showed efficient recovery: total phenolic content (28.4 and23.2 mg GAE/g), total flavonoids content (44.8 and 34.5 mg RE/g), total anthocyanins content (4.1 and 16.3mg Cy-3-glu/100 g) and extraction yields (29.5 and 23.2 %) and remarkable antioxidant activity(IC50=12.35 and 15.76 mg L-1) for yellow and red petal extracts, respectively. Main compounds werecyanidin-3-sophoroside and cyanidin-3,5-diglucoside, besides catechin, epicatechin and rutin. The extractswere classified as non-irritating ingredients. The data revealed HRS as a source of bioactive compounds and significant variations in phytochemical content among yellow and red petals of HRS.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
