Objective: A hernia of the abdominal wall is an opening of the muscles in the abdominal wall, which is frequently treated via the application of a surgical mesh. The purpose of this research is to study how human adipose-derived stem cells (hADSCs) interact with Phasix™ Mesh, a commercially available mesh for hernia repair. Studying how cells derived from the abdominal region behave with Phasix™ Mesh is crucial to improve the state of the art of current surgery and achieve effective tissue restoration. Materials and methods: hADSCs were seeded onto Phasix™ Mesh, a fully resorbable surgical mesh of poly (4-hydroxybutyric acid) (P4HB). Cell viability was assessed through MTT assay, and cell growth and adhesion were evaluated via multiple imaging techniques and gene imaging profiling. Results: Results confirm that the nets support cells proliferation, extracellular matrix production and increasing of angiogenetic factor. Conclusions: Butyric acid-based nets are promising scaffolds for abdominal wall reconstruction.

Tissue reconstruction of abdominal wall with butyric acid-based nets: preliminary in vitro test using tissue engineering strategies

Zanolla, I
Primo
Investigation
;
Tiengo, E
Secondo
Methodology
;
Zanotti, F
Methodology
;
Trentini, M
Investigation
;
Soliani, G
Penultimo
Supervision
;
Zavan, B
Ultimo
Conceptualization
2022

Abstract

Objective: A hernia of the abdominal wall is an opening of the muscles in the abdominal wall, which is frequently treated via the application of a surgical mesh. The purpose of this research is to study how human adipose-derived stem cells (hADSCs) interact with Phasix™ Mesh, a commercially available mesh for hernia repair. Studying how cells derived from the abdominal region behave with Phasix™ Mesh is crucial to improve the state of the art of current surgery and achieve effective tissue restoration. Materials and methods: hADSCs were seeded onto Phasix™ Mesh, a fully resorbable surgical mesh of poly (4-hydroxybutyric acid) (P4HB). Cell viability was assessed through MTT assay, and cell growth and adhesion were evaluated via multiple imaging techniques and gene imaging profiling. Results: Results confirm that the nets support cells proliferation, extracellular matrix production and increasing of angiogenetic factor. Conclusions: Butyric acid-based nets are promising scaffolds for abdominal wall reconstruction.
2022
Zanolla, I; Tiengo, E; Zanotti, F; Trentini, M; Mortellaro, C; Lucchina, A G; Licastro, D; Dal Monego, S; Soliani, G; Zavan, B
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/2531097
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