The impact of two different stationary phases and ion-pair reagents on the retention behavior of glucagon, a therapeutic peptide consisting of 29 amino acidic residues, has been investigated under reversed-phase elution conditions. Retention of glucagon was investigated under isocratic conditions by varying the fraction of the organic modifier in the range of 28–38% (v/v). The two stationary phases have been characterized in terms of excess adsorption isotherms to understand the preferential adsorption of eluent components on them. Results suggest that the ligand characteristics and the pH of the mobile phase play a pivotal role on retention.

The Role of Adsorption and pH of the Mobile Phase on the Chromatographic Behavior of a Therapeutic Peptide

Simona Felletti
Primo
;
Chiara De Luca
Secondo
;
Giulio Lievore;Alessandro Buratti;Desiree Bozza;Alberto Cavazzini
Penultimo
;
Martina Catani
Ultimo
2021

Abstract

The impact of two different stationary phases and ion-pair reagents on the retention behavior of glucagon, a therapeutic peptide consisting of 29 amino acidic residues, has been investigated under reversed-phase elution conditions. Retention of glucagon was investigated under isocratic conditions by varying the fraction of the organic modifier in the range of 28–38% (v/v). The two stationary phases have been characterized in terms of excess adsorption isotherms to understand the preferential adsorption of eluent components on them. Results suggest that the ligand characteristics and the pH of the mobile phase play a pivotal role on retention.
2021
Felletti, Simona; DE LUCA, Chiara; Lievore, Giulio; Buratti, Alessandro; Bozza, Desiree; Macis, Marco; Ricci, Antonio; Cabri, Walter; Cavazzini, Alber...espandi
File in questo prodotto:
File Dimensione Formato  
Felletti2021_The_role_of_adsorption_and_pH_of_the_mobile_phase_on_the_chrom_behavior_of_a_therapeutic_peptide-felletti_2021_LCGC (1).pdf

accesso aperto

Descrizione: Articolo
Tipologia: Full text (versione editoriale)
Licenza: PUBBLICO - Pubblico con Copyright
Dimensione 1.97 MB
Formato Adobe PDF
1.97 MB Adobe PDF Visualizza/Apri
LCGC_NASupp_June2021 (1).pdf

accesso aperto

Descrizione: Full text editoriale
Tipologia: Full text (versione editoriale)
Licenza: PUBBLICO - Pubblico con Copyright
Dimensione 10.97 MB
Formato Adobe PDF
10.97 MB Adobe PDF Visualizza/Apri

I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/2509571
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact