Over the last decade, several cryogenic modulators have been developed, and found application in the comprehensive two-dimensional gas chromatography (GC×GC) field. Although the effectiveness of such a modulation approach is not under discussion, it is also a rather expensive issue. Consequently, the concept and development of low-costing flow modulation methods is of high interest. The present lecture is focused on the development and use of a highly flexible, chip-based flow modulator for comprehensive two-dimensional gas chromatography. The novel modulation system consists of a metallic disc (chip), with internal channels, located entirely within the GC oven; apart from the two dimensions, the disc is linked to an auxiliary pressure unit, and is characterized by an interchangeable sample loop, which simplifies the optimization step and maximizes the overall GC×GC performance. The valve part of the modulator is located outside the GC oven, and, hence, there are no temperature restrictions. A series of flow-modulated GC×GC-MS applications will be shown, relative to a variety of complex real-world samples. The data processing step was simplified by use of a novel, user-friendly comprehensive chromatography software.
Flow-modulated Comprehensive 2D Gas Chromatography-Mass Spectrometry assisted by a novel data-processing software
F. Franchina;
2012
Abstract
Over the last decade, several cryogenic modulators have been developed, and found application in the comprehensive two-dimensional gas chromatography (GC×GC) field. Although the effectiveness of such a modulation approach is not under discussion, it is also a rather expensive issue. Consequently, the concept and development of low-costing flow modulation methods is of high interest. The present lecture is focused on the development and use of a highly flexible, chip-based flow modulator for comprehensive two-dimensional gas chromatography. The novel modulation system consists of a metallic disc (chip), with internal channels, located entirely within the GC oven; apart from the two dimensions, the disc is linked to an auxiliary pressure unit, and is characterized by an interchangeable sample loop, which simplifies the optimization step and maximizes the overall GC×GC performance. The valve part of the modulator is located outside the GC oven, and, hence, there are no temperature restrictions. A series of flow-modulated GC×GC-MS applications will be shown, relative to a variety of complex real-world samples. The data processing step was simplified by use of a novel, user-friendly comprehensive chromatography software.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
