Background: Killian polyp (KP) is a benign lesion that arises from the maxillary sinus. The etiology of KP is unknown. The aim of this study was to investigate the potential involvement of human papilloma- (HPV) and polyoma-viruses (HPyV) infections in the onset of KP. Methods: DNA from antral (n = 14) and nasal (n = 14) KP fractions were analyzed for HPV and HPyV sequences, genotypes, viral DNA load and physical status along with expression of viral proteins and p16 cellular protein. Results: The oncogenic HPV16 was detected in 3/14 (21.4%) antral KPs, whilst nasal KPs tested HPV-negative (0/14). The mean HPV16 DNA load was 4.65 ± 2.64 copy/104 cell. The whole HPV16 episomal genome was detected in one KP sample, whereas HPV16 DNA integration in two KPs. P16 mRNA level was lower in the KP sample carrying HPV16 episome than in KPs carrying integrated HPV16 and HPV- negative KPs (p< 0.001). None of the antral and nasal KP samples tested positive for HPyV DNA (0/28). Conclusions: A fraction of KP tested positive for the oncogenic HPV16. HPV16 detection in the KP antral portion may be consistent with HPV16 infection derived from the maxillary sinus. HPV16 DNA integration represents a novel finding. Altogether, these data improve our knowledge on the association between KP and HPV infection, whereas it indicates that the KP onset is heterogeneous.

Association between oncogenic human papillomavirus type 16 and Killian polyp

Lucia Oton-Gonzalez
Co-primo
Writing – Original Draft Preparation
;
John Charles Rotondo
Co-primo
Writing – Review & Editing
;
Luca Cerritelli
Resources
;
Nicola Malagutti
Membro del Collaboration Group
;
Carmen Lanzillotti
Data Curation
;
Ilaria Bononi
Validation
;
Andrea Ciorba
Methodology
;
Chiara Bianchini;Chiara Mazziotta
Investigation
;
Monica De Mattei
Software
;
Stefano Pelucchi
Visualization
;
Mauro Tognon
Penultimo
Supervision
;
Fernanda Martini
Ultimo
Conceptualization
2021

Abstract

Background: Killian polyp (KP) is a benign lesion that arises from the maxillary sinus. The etiology of KP is unknown. The aim of this study was to investigate the potential involvement of human papilloma- (HPV) and polyoma-viruses (HPyV) infections in the onset of KP. Methods: DNA from antral (n = 14) and nasal (n = 14) KP fractions were analyzed for HPV and HPyV sequences, genotypes, viral DNA load and physical status along with expression of viral proteins and p16 cellular protein. Results: The oncogenic HPV16 was detected in 3/14 (21.4%) antral KPs, whilst nasal KPs tested HPV-negative (0/14). The mean HPV16 DNA load was 4.65 ± 2.64 copy/104 cell. The whole HPV16 episomal genome was detected in one KP sample, whereas HPV16 DNA integration in two KPs. P16 mRNA level was lower in the KP sample carrying HPV16 episome than in KPs carrying integrated HPV16 and HPV- negative KPs (p< 0.001). None of the antral and nasal KP samples tested positive for HPyV DNA (0/28). Conclusions: A fraction of KP tested positive for the oncogenic HPV16. HPV16 detection in the KP antral portion may be consistent with HPV16 infection derived from the maxillary sinus. HPV16 DNA integration represents a novel finding. Altogether, these data improve our knowledge on the association between KP and HPV infection, whereas it indicates that the KP onset is heterogeneous.
OTON GONZALEZ, Lucia; Rotondo, John Charles; Cerritelli, Luca; Malagutti, Nicola; Lanzillotti, Carmen; Bononi, Ilaria; Ciorba, Andrea; Bianchini, Chiara; Mazziotta, Chiara; DE MATTEI, Monica; Pelucchi, Stefano; Tognon, Mauro; Martini, Fernanda
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11392/2442189
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