For many archaeological and paleontological samples, the relative content of endogenous compared to contaminant DNA is low. In such cases, enriching sequencing libraries for endogenous DNA, prior to sequencing can make the final research project more cost-effective. Here, we present an in-solution enrichment protocol based on homemade baits that can be applied to recover complete nuclear genomes from ancient remains. The approach is based on the preparation of DNA baits by biotinylated adapter ligation. The procedure has been developed for use with human remains but can be adapted to other species or target regions by choosing the appropriate template DNA from which to build the capture baits. By using homemade rather than commercially acquired baits, this protocol may offer increased flexibility and cost efficiency.
|Titolo:||Whole-genome capture of ancient DNA using homemade baits|
GONZALEZ FORTES, Gloria Maria (Primo) (Corresponding)
|Data di pubblicazione:||2019|
|Appare nelle tipologie:||02.1 Contributo in volume (Capitolo, articolo)|