Background Primary non-ampullary small bowel adenocarcinoma (SBAs) is a remarkably rare tumor in the general population. Celiac disease (CD) is associated with an increased risk of developing SBA. Published data on the morphologic, phenotypic and molecular features of CD-associated SBAs (CD-SBAs) are very limited. Design We analyzed 11 SBAs arisen in the setting of CD collected from four different Italian Institutions. Immunoreactions for intestinal markers (MUC2, CDX2, CD10) and for gastric markers (MUC5AC, MUC6) were performed on representative sections of each SBA. In all cases, non-tumoral small intestinal mucosa was also examined. Additionally, we investigated the frequency of KRAS, NRAS, and BRAF somatic mutations in 9 out of 11 cases. Results The average age of patients was 56 years (rang 38-72) with predilection of females (9 females and 2 males). Three tumors were at stage III or IV and 1 patient died of disease after 6 months from diagnosis. One tumor was localized in the duodenum (second part), the remaining ten were in the jejunum. On histologic examination, three neoplasms were high grade poorly differentiated carcinomas (grade 3), one of which showing a poorly cohesive component with signet ring cells, while 8 were low-grade intestinal-type tubular adenocarcinomas. Ten cases were positive for CDX2, 7 for MUC2 and 7 for CD10. One case showed >5% MUC5AC-positive cells. No case had MUC6. Sequencing analysis of KRAS, NRAS, and BRAF hot spot regions revealed the presence of mutations only at codon 12 in exon 2 of KRAS in 7 of 9 cases. We found no mutations in the hot spot regions of NRAS (exons 2, 3, 4) or BRAF (exon 15). Conclusion CD-SBAs appear to have a predilection for younger people (especially female) than other SBAs and may behave aggressively. Most CD-SBAs express structural and phenotypic patterns of intestinal differentiation, including CD10 (a marker of the enterocyte brush border). In about 30% of cases they can show a high grade histology. Unlike SBAs associated with Crohn’s disease, CD-SBAs seem to rarely express gastric markers.

Celiac Disease-Associated Small Bowel Adenocarcinomas: Clinico-Pathologic, Phenotypic and Molecular Study of 11 Cases

Caio, G;
2015

Abstract

Background Primary non-ampullary small bowel adenocarcinoma (SBAs) is a remarkably rare tumor in the general population. Celiac disease (CD) is associated with an increased risk of developing SBA. Published data on the morphologic, phenotypic and molecular features of CD-associated SBAs (CD-SBAs) are very limited. Design We analyzed 11 SBAs arisen in the setting of CD collected from four different Italian Institutions. Immunoreactions for intestinal markers (MUC2, CDX2, CD10) and for gastric markers (MUC5AC, MUC6) were performed on representative sections of each SBA. In all cases, non-tumoral small intestinal mucosa was also examined. Additionally, we investigated the frequency of KRAS, NRAS, and BRAF somatic mutations in 9 out of 11 cases. Results The average age of patients was 56 years (rang 38-72) with predilection of females (9 females and 2 males). Three tumors were at stage III or IV and 1 patient died of disease after 6 months from diagnosis. One tumor was localized in the duodenum (second part), the remaining ten were in the jejunum. On histologic examination, three neoplasms were high grade poorly differentiated carcinomas (grade 3), one of which showing a poorly cohesive component with signet ring cells, while 8 were low-grade intestinal-type tubular adenocarcinomas. Ten cases were positive for CDX2, 7 for MUC2 and 7 for CD10. One case showed >5% MUC5AC-positive cells. No case had MUC6. Sequencing analysis of KRAS, NRAS, and BRAF hot spot regions revealed the presence of mutations only at codon 12 in exon 2 of KRAS in 7 of 9 cases. We found no mutations in the hot spot regions of NRAS (exons 2, 3, 4) or BRAF (exon 15). Conclusion CD-SBAs appear to have a predilection for younger people (especially female) than other SBAs and may behave aggressively. Most CD-SBAs express structural and phenotypic patterns of intestinal differentiation, including CD10 (a marker of the enterocyte brush border). In about 30% of cases they can show a high grade histology. Unlike SBAs associated with Crohn’s disease, CD-SBAs seem to rarely express gastric markers.
Vanoli, A; Di Sabatino, A; Manca, R; Grillo, F; Volta, U; Santini, D; Elli, L; Ferrero, S; Viglio, A; Riboni, R; Dallera, E; Arra, M; Rappa, A; Caio, G; Luinetti, O; Solcia, E; Pauli, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/2407748
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