The genus Echinacea (Asteraceae) includes species traditionally used in phytotherapy. Among them, Echinacea pallida (Nutt.) Nutt. root extracts are characterized by a representative antiproliferative activity, due to the presence of acetylenic compounds. In this study, supercritical fluid extraction (SFE) was applied and compared with conventional Soxhlet extraction (SE) in order to obtain a bioactive extract highly rich in polyacetylenes and polyenes from E. pallida roots. The composition of the extracts was monitored by means of HPLCâUV/DAD and HPLCâESIâMSn by using an Ascentis Express C18 column (150 mm à 3.0 mm I.D., 2.7 μm, Supelco, Bellefonte, PA, USA) with a mobile phase composed of (A) water and (B) acetonitrile, under gradient elution. By keeping SFE time at the threshold of 1 h (15 min static and 45 min dynamic for 1 cycle) with the oven temperature set at 40â45 °C and 90 bar of pressure, an overall extraction yield of 1.18â1.21% (w/w) was obtained, with a high selectivity for not oxidized lipophilic compounds. The biological activity of the extracts was evaluated against human non-small lung A549 and breast carcinoma MCF-7 cancer cell lines. The cytotoxic effect of the SFE extract was more pronounced towards the MCF-7 than the A549 cancer cells, with IC50 values ranging from 21.01 ± 2.89 to 31.11 ± 2.l4 μg/mL; cell viability was affected mainly between 24 and 48 h of exposure. The results show the possibility of a new âgreenâ approach to obtain extracts highly rich in genuine polyacetylenes and polyenes from E. pallida roots. The bioactivity evaluation confirmed the cytotoxicity of E. pallida extracts against the considered cancer cell lines, especially against MCF-7 cells, thus suggesting to represent a valuable tool for applicative purposes in cancer prevention.
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Data di pubblicazione: | 2017 | |
Titolo: | A new method based on supercritical fluid extraction for polyacetylenes and polyenes from Echinacea pallida (Nutt.) Nutt. roots | |
Autori: | Tacchini, Massimo; Spagnoletti, Antonella; Brighenti, Virginia; Prencipe, Francesco Pio; Benvenuti, Stefania; Sacchetti, Gianni; Pellati, Federica | |
Rivista: | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | |
Keywords: | Anticancer activity; Echinacea pallida; HPLC; Polyacetylenes and polyenes; Supercritical fluid extraction; Analytical Chemistry; 3003; Drug Discovery3003 Pharmaceutical Science; Spectroscopy; Clinical Biochemistry | |
Abstract in inglese: | The genus Echinacea (Asteraceae) includes species traditionally used in phytotherapy. Among them, Echinacea pallida (Nutt.) Nutt. root extracts are characterized by a representative antiproliferative activity, due to the presence of acetylenic compounds. In this study, supercritical fluid extraction (SFE) was applied and compared with conventional Soxhlet extraction (SE) in order to obtain a bioactive extract highly rich in polyacetylenes and polyenes from E. pallida roots. The composition of the extracts was monitored by means of HPLCâUV/DAD and HPLCâESIâMSn by using an Ascentis Express C18 column (150 mm à 3.0 mm I.D., 2.7 μm, Supelco, Bellefonte, PA, USA) with a mobile phase composed of (A) water and (B) acetonitrile, under gradient elution. By keeping SFE time at the threshold of 1 h (15 min static and 45 min dynamic for 1 cycle) with the oven temperature set at 40â45 °C and 90 bar of pressure, an overall extraction yield of 1.18â1.21% (w/w) was obtained, with a high selectivity for not oxidized lipophilic compounds. The biological activity of the extracts was evaluated against human non-small lung A549 and breast carcinoma MCF-7 cancer cell lines. The cytotoxic effect of the SFE extract was more pronounced towards the MCF-7 than the A549 cancer cells, with IC50 values ranging from 21.01 ± 2.89 to 31.11 ± 2.l4 μg/mL; cell viability was affected mainly between 24 and 48 h of exposure. The results show the possibility of a new âgreenâ approach to obtain extracts highly rich in genuine polyacetylenes and polyenes from E. pallida roots. The bioactivity evaluation confirmed the cytotoxicity of E. pallida extracts against the considered cancer cell lines, especially against MCF-7 cells, thus suggesting to represent a valuable tool for applicative purposes in cancer prevention. | |
Digital Object Identifier (DOI): | 10.1016/j.jpba.2017.07.053 | |
Handle: | http://hdl.handle.net/11392/2382687 | |
Appare nelle tipologie: | 03.1 Articolo su rivista |