Human leukocyte antigen (HLA)-F has been involved in immune regulation of infectious diseases. However, the role of HLA-F polymorphisms in hepatitis B infection outcomes remains unclear. Here, we aimed to determine HLA-F polymorphism implication in chronic HBV. Genotype analysis was performed for three single nucleotide polymorphisms (SNPs) of HLA-F and one SNP of HLA-E using PCR-SSP, in 252 Tunisian patients with chronic HBV infection stratified according to their HBV DNA levels (140 patients with low HBV DNA levels < 2000 IU/mL and 112 patients with high HBV DNA levels >= 2000 IU/mL) and 240 healthy controls (CTRL). The three HLA-F SNPs (HLA-F* 01:02, -F* 01:03 and -F* 01:04) have the same allelic and genotypic frequencies in patients and in CTRL. We reported a low HLA-F* 01:02 and -F*01:04 allelic frequencies in the Tunisian population; however, high HLA-F* 01:03 allele frequencies were observed (17%). A significant association was found between the HLA-F* 01:03 allele and decreased level of HBV DNA (P = 0.02 OR 0.56, 95% CI 0.35-0.92). No significant differences were observed in haplotype distribution between patients and CTRL. A significant association of HLA-F* 01:03 with the level of HBV DNA suggests an important role of HLA-F in HBV replication control.

Human leukocyte antigen (HLA-F) polymorphism is associated with chronic HBV infection

Roberta Rizzo
Penultimo
;
2018

Abstract

Human leukocyte antigen (HLA)-F has been involved in immune regulation of infectious diseases. However, the role of HLA-F polymorphisms in hepatitis B infection outcomes remains unclear. Here, we aimed to determine HLA-F polymorphism implication in chronic HBV. Genotype analysis was performed for three single nucleotide polymorphisms (SNPs) of HLA-F and one SNP of HLA-E using PCR-SSP, in 252 Tunisian patients with chronic HBV infection stratified according to their HBV DNA levels (140 patients with low HBV DNA levels < 2000 IU/mL and 112 patients with high HBV DNA levels >= 2000 IU/mL) and 240 healthy controls (CTRL). The three HLA-F SNPs (HLA-F* 01:02, -F* 01:03 and -F* 01:04) have the same allelic and genotypic frequencies in patients and in CTRL. We reported a low HLA-F* 01:02 and -F*01:04 allelic frequencies in the Tunisian population; however, high HLA-F* 01:03 allele frequencies were observed (17%). A significant association was found between the HLA-F* 01:03 allele and decreased level of HBV DNA (P = 0.02 OR 0.56, 95% CI 0.35-0.92). No significant differences were observed in haplotype distribution between patients and CTRL. A significant association of HLA-F* 01:03 with the level of HBV DNA suggests an important role of HLA-F in HBV replication control.
2018
Baligh Laaribi, Ahmed; Hannachi, Naila; Ben Yahia, Hamza; Marzouk, Manal; Mehri, Asma; Belhadj, Manel; Yacoub, Salwa; Letaief, Amel; Ouzari, Hadda-Imene; Boudabous, Abdellatif; Boukadida, Jalel; Rizzo, Roberta; Zidi, Inès
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/2381338
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