Define a common methodology for the geometric memory surveying of all the architectures ascribed to Leon Battista Alberti and the creation of the related morphometric database was the challenge that led the DIAPReM center of the University of Ferrara to carry out an intensive survey campaign in collaboration with the Centro Studi Leon Battista Alberti since 2005. The integration of topographic techniques and three-dimensional laser scanners surveying methodologies allowed the creation of a 3D high-density morphometric database with possibilities of query, implementation and navigation, even on the Web, aimed at research, conservation and dissemination purposes. The architectural interpretation of San Sebastiano in Mantua in relation to existing surveys is an example of Alberti's work critical analysis, implemented thanks to the generated models database. The methodological organization of the models allows to read previous surveys more accurately and to perform further analysis, like monitoring for building management and preservation, through a structured comparison logic. The same methodological framework was the key to analyze the morphology of S.Sebastiano starting from the drawing of Antonio Labacco.